Adsorption of human IgG on to poly(N-isopropylacrylamide)-based polymer particles

被引：0
|
作者：
Claudia S. O. Silva
Ricardo P. Baptista
Andrea M. Santos
José M. G. Martinho
Joaquim M. S. Cabral
M. Ângela Taipa
机构：
[1] Instituto Superior Técnico,Centro de Engenharia Biológica e Química
[2] Instituto Superior Técnico,Centro de Química
来源：
Biotechnology Letters | 2006年 / 28卷
关键词：
Adsorption; Human immunoglobulin G; Poly(methyl methacrylate); Poly(; -isopropylacrylamide); Thermosensitive polymers;
D O I：
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学科分类号：
摘要：
Thermosensitive poly(N-isopropylacrylamide)-based polymer particles were synthesised, and screened for the adsorption of human immunoglobulin G (hIgG). At pH 9 the adsorption on microgel particles was strongly affected by temperature, approximately 40 mg hIgG/g support (90% of initial hIgG) being adsorbed at 40°C but only 10% of initial hIgG at 25°C. At pH 5 the maximum adsorbed amount (20 mg hIgG/g support) was similar for both temperatures. The adsorption of hIgG on to charged poly(methyl methacrylate)/poly(N-isopropylacrylamide) core-shell latexes was negligible (5–10 mg hIgG/g support) at the same temperature and pH conditions. The lower adsorption of hIgG onto the core-shell particles is explained by steric interactions due to the small size of the shell.
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页码：2019 / 2025
页数：6
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