Replacing critical point drying with a low-cost chemical drying provides comparable surface image quality of glandular trichomes from leaves of Millingtonia hortensis L. f. in scanning electron micrograph

被引：35

作者：

Bhattacharya R. ^{[1
]}

Saha S. ^{[1
]}

Kostina O. ^{[2
]}

Muravnik L. ^{[2
]}

Mitra A. ^{[1
]}

机构：

[1] Natural Product Biotechnology Group, Agricultural and Food Engineering Department, Indian Institute of Technology Kharagpur, Kharagpur

[2] Laboratory of Plant Anatomy and Morphology, Komarov Botanical Institute, Russian Academy of Sciences, Professor Popov Street 2, Saint Petersburg

来源：

Applied Microscopy | 2020年 / 50卷 / 01期

基金：

俄罗斯基础研究基金会;

关键词：

Chemical drying; Critical-point drying; Glandular trichomes; Hexamethyldisilazane; Millingtonia hortensis; Scanning electron microscope;

D O I：

10.1186/s42649-020-00035-6

中图分类号：

O61 [无机化学]; TQ [化学工业];

学科分类号：

070301 ; 0817 ; 081704 ;

摘要：

Sample preparation including dehydration and drying of samples is the most intricate part of scanning electron microscopy. Most current sample preparation protocols use critical-point drying with liquid carbon dioxide. Very few studies have reported samples that were dried using chemical reagents. In this study, we used hexamethyldisilazane, a chemical drying reagent, to prepare plant samples. As glandular trichomes are among the most fragile and sensitive surface structures found on plants, we used Millingtonia hortensis leaf samples as our study materials because they contain abundant glandular trichomes. The results obtained using this new method are identical to those produced via critical-point drying. © 2020, The Author(s).

引用

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