Genetic and serological characterization of chrysanthemum stem necrosis virus, a member of the genus Tospovirus

被引：0
作者：
Pei-Ru Wu
Wan-Chu Chien
Mitsuru Okuda
Minoru Takeshita
Shyi-Dong Yeh
Yun-Chi Wang
Tsung-Chi Chen
机构：
[1] Asia University,Department of Biotechnology
[2] National Agriculture and Food Research Organization,Agricultural Research Center
[3] Kyushu University,Laboratory of Plant Pathology, Faculty of Agriculture
[4] National Chung Hsing University,Department of Plant Pathology
[5] National Chung Hsing University,Agricultural Biotechnology Center
[6] China Medical University Hospital,Department of Medical Research
[7] China Medical University,undefined
来源：
Archives of Virology | 2015年 / 160卷
关键词：
Tomato Spot Wilt Virus; Spot Virus; Iris Yellow Spot Virus; Impatiens Necrotic Spot Virus; Melon Yellow Spot Virus;
D O I：
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中图分类号：
学科分类号：
摘要：
Chrysanthemum stem necrosis virus (CSNV) is a member of a tentative tospovirus species. In this study, the complete genomic sequence of the Japanese CSNV isolate TcCh07A was determined. The L RNA is 8960 nt long and encodes the 331.0-kDa RNA-dependent RNA polymerase. The M RNA is 4828 nt long and encodes the 34.1-kDa movement protein (NSm) and the 127.7-kDa glycoprotein precursor (Gn/Gc). The S RNA is 2949 nt long and encodes the 52.4-kDa silencing suppressor protein (NSs) and the 29.3-kDa nucleocapsid (N) protein. The N protein of CSNV-TcCh07A was purified from virus-infected plant tissues and used for production of a rabbit polyclonal antiserum (RAs) and a monoclonal antibody (MAb). Results of serological tests by indirect ELISA and western blotting using the prepared RAs and MAb and a previously produced RAs against the N protein of tomato spotted wilt virus (TSWV) indicated that CSNV-TcCh07A, TSWV, tomato chlorotic spot virus, groundnut ringspot virus, alstroemeria necrotic streak virus and impatiens necrotic spot virus are serologically related.
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页码：529 / 536
页数：7
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