Recombinase Polymerase Amplification for Rapid Detection of Human Bacterial Pneumonia Pathogens

被引：0
作者：
S. A. Lapa
S. A. Surzhikov
S. A. Blagodatskikh
V. E. Shershov
A. V. Chudinov
机构：
[1] Engelhardt Institute of Molecular Biology,
[2] Russian Academy of Sciences,undefined
[3] Scientific Center of Applied Microbiology and Biotechnology,undefined
来源：
Molecular Biology | 2023年 / 57卷
关键词：
recombinase polymerase amplification; RPA; bacterial pneumonia;
D O I：
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学科分类号：
摘要：
A diagnostic system based on recombinase polymerase amplification (RPA) has been developed to identify six bacterial pathogens of human pneumonia. Species-specific primers have been designed and optimized to conduct a multiplex reaction in one common volume. Labeled primers were used for reliable discrimination of amplification products that are similar in size. Identification of the pathogen was carried out by visual analysis of an electrophoregram. The analytical sensitivity of the developed multiplex RPA was 102–103 copies of DNA. The specificity of the system was determined by the absence of cross-amplification of the studied DNA samples of pneumonia pathogens for each pair of primers, as well as for the DNA of Mycobacterium tuberculosis H37rv, and amounted to 100%. The execution time of the analysis is less than an 1 h, including the electrophoretic reaction control. The test system can be used in specialized clinical laboratories for rapid analysis of samples from patients with suspected pneumonia.
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页码：544 / 549
页数：5
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