Diagnosis of HEV infection by serological and real-time PCR assays: A study on acute non-A-C hepatitis collected from 2004 to 2010 in Italy

被引：19

作者：

Angela Candido

Stefania Taffon

Paola Chionne

Giulio Pisani

Elisabetta Madonna

Stefano Dettori

Abir Hamza

Catia Valdarchi

Roberto Bruni

Anna Rita Ciccaglione

机构：

[1] Department of Infectious, Parasitic and Immune-Mediated Diseases, Istituto Superiore di Sanità, Rome

[2] Center for Immunobiologicals Research and Evaluation, Istituto Superiore di Sanità, Rome

[3] Laboratory of Transmissible Illness and Active Biological Substances, Faculty of Pharmacy, University of Monastir, Monastir

来源：

BMC Research Notes | / 5卷 / 1期

关键词：

Infectious Disease Unit; Sporadic Acute Hepatitis; Acute Hepatitis Case; Beijing Wantai Biological Pharmacy Enterprise; Current Serological Assay;

D O I：

10.1186/1756-0500-5-297

中图分类号：

学科分类号：

摘要：

Background: The impact of hepatitis E in developed countries, like Italy, still requires a clear definition. In the present study, we evaluated HEV infection in patients with acute non-A-C hepatitis by an approach comparing data from Real-time PCR and serological assays. Methods. In a first analysis, sera from 52 patients hospitalized with a diagnosis of acute viral non-A-C hepatitis in Italy were tested by in-house Real-Time PCR assay for identification of Hepatitis E Virus (HEV) RNA and by anti-HEV IgM and IgG assays. In a subsequent analysis, selected samples were evaluated by additional IgM tests to confirm diagnosis. Results: Among the 52 samples, 21 showed positive results for all three markers (IgM, IgG and HEV RNA). One patient showed HEV RNA as single marker. Uncertain results were found in 8 samples while the remaining 22 were negative for all markers. Further analysis of the 8 undefined samples by additional IgM tests confirmed HEV infection in 1 patient. Overall, acute HEV infections were reliably identified in 23 (44.2%) out of 52 patients. Conclusions: In the present paper, we performed a study evaluating HEV infection in 52 sporadic non-A-C acute hepatitis cases. All samples were collected from 2004 to 2010 in Italy. By a diagnostic strategy based on genomic and serological assays we identified HEV infections in 23 out of 52 patients (44.2%), a percentage higher than previous estimates. Thus, the actual impact of HEV infections in Italy needs to be further evaluated on a national scale by a diagnostic strategy based on multiple and last generation assays. © 2012 Candido et al.

引用

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