Green Synthesis of Silver Nanoparticles Using Mushroom Extract of Pleurotus giganteus: Characterization, Antimicrobial, and α-Amylase Inhibitory Activity

被引:0
|
作者
Gopal Debnath
Panna Das
Ajay Krishna Saha
机构
[1] Tripura University,Mycology and Plant pathology Laboratory, Department of Botany
[2] Tripura University,Microbiology Laboratory, Department of Botany
来源
BioNanoScience | 2019年 / 9卷
关键词
Green synthesis; Silver nanoparticles; Antimicrobial activity; α-Amylase inhibition assay;
D O I
暂无
中图分类号
学科分类号
摘要
The present study was carried out to synthesize silver nanoparticles (AgNPs) using extracts of wild edible mushroom, Pleurotus giganteus, and to characterize the synthesized AgNPs and also to evaluate the synthesized AgNPs for antimicrobial and α-amylase inhibitory activity. Green synthesized AgNPs were characterized by UV–Vis spectroscopy, Fourier-transform infrared (FTIR) spectroscopy, Atomic force microscopy (AFM), Transmission electron microscopy (TEM), Scanning electron microscopy (SEM), selected area electron diffraction (SAED), Energy dispersive X-ray spectroscopy (EDX), and X-ray diffraction (XRD). Antibacterial activity of AgNPs was studied by disc diffusion method along with minimum inhibitory concentration (MIC) and α-amylase inhibition assay of AgNPs were also studied. UV–Vis spectra of reaction mixture of AgNPs exhibited surface plasmon resonance peak around at 420 nm. FTIR study revealed that mainly carboxyl, hydroxyl, and amine functional groups were present in a mushroom extract which mainly reduced Ag+ to Ag0. AFM, TEM, SEM, SAED, and XRD pattern analysis supported that synthesized AgNPs were mostly spherical shaped within average size 2–20 nm and crystalline in nature. Biosynthesized AgNPs showed more antibacterial potentiality against Gram (–) bacteria. MIC of green synthesized AgNPs were found against Escherichia coli, Pseudomonas aeruginosa, Bacillus subtilis, and Staphylococcus aureus as 12, 10, 14, and 15 μg/ml, respectively. α-Amylase inhibition assay of green synthesized AgNPs revealed that percent inhibition of α-amylase decreased with increasing concentration of green synthesized AgNPs. Wild edible mushroom P. giganteus can be used as a source of reducing and capping agents of spherical metallic silver nanoparticles which offers potential medicinal properties.
引用
收藏
页码:611 / 619
页数:8
相关论文
共 50 条
  • [1] Green Synthesis of Silver Nanoparticles Using Mushroom Extract of Pleurotus giganteus: Characterization, Antimicrobial, and α-Amylase Inhibitory Activity
    Debnath, Gopal
    Das, Panna
    Saha, Ajay Krishna
    BIONANOSCIENCE, 2019, 9 (03) : 611 - 619
  • [2] Characterization, Antimicrobial and α-Amylase Inhibitory Activity of Silver Nanoparticles Synthesized by using Mushroom Extract of Lentinus tuber-regium
    Debnath G.
    Das P.
    Saha A.K.
    Proceedings of the National Academy of Sciences, India Section B: Biological Sciences, 2020, 90 (1) : 37 - 45
  • [3] Green synthesis of silver nanoparticles using green tea leaf extract, characterization and evaluation of antimicrobial activity
    Widatalla, Hiba Abbas
    Yassin, Layla Fathi
    Alrasheid, Ayat Ahmed
    Ahmed, Shimaa Abdel Rahman
    Widdatallah, Marvit Osman
    Eltilib, Sahar Hussein
    Mohamed, Alaa Abdulmoneim
    NANOSCALE ADVANCES, 2022, 4 (03): : 911 - 915
  • [4] Green Synthesis, Characterization and Antimicrobial Activity of Silver Nanoparticles Using Uraria picta Leaves Extract
    Mishra S.
    Kumavat S.
    Micro and Nanosystems, 2022, 14 (03) : 212 - 225
  • [5] Green synthesis of silver nanoparticles using tree oyster mushroom Pleurotus ostreatus and its inhibitory activity against pathogenic bacteria
    Al-Bahrani, Rusol
    Raman, Jegadeesh
    Lakshmanan, Hariprasath
    Hassan, Abdullah A.
    Sabaratnam, Vikineswary
    MATERIALS LETTERS, 2017, 186 : 21 - 25
  • [6] Green Synthesis of Silver Nanoparticles Using Mushroom Species, Their Characterization and Catalytic Activity
    Bhangale, H. G.
    Bachhav, S. G.
    Sarode, K. M.
    Patil, D. R.
    TECHNO-SOCIETAL 2018: PROCEEDINGS OF THE 2ND INTERNATIONAL CONFERENCE ON ADVANCED TECHNOLOGIES FOR SOCIETAL APPLICATIONS - VOL 2, 2020, : 329 - 335
  • [7] Green Synthesis, Characterization and In Vitro Antimicrobial Activity of Silver Nanoparticles (AgNPs) Using Fungal Aqueous Extract
    War, John Mohd
    Wani, Abdul Hamid
    Nisa, Anees Un
    Bhat, Mohd Yaqub
    NANO, 2022, 17 (13)
  • [8] Green Synthesis, Characterization and Antimicrobial Activity of Silver Nanoparticles from the Extract of Lagerstroemia speciosa
    Prakash, Vinit
    Kaur, Harpreet
    Kumari, Anjana
    Kumar, Manoj
    Gupta, Sumeet
    Bala, Ritu
    ORIENTAL JOURNAL OF CHEMISTRY, 2021, 37 (03) : 648 - 655
  • [9] Green Synthesis and Antimicrobial Activity of Monodispersed Silver Nanoparticles Synthesized Using Lemon Extract
    Selvakumar, P. Mosae
    Antonyraj, Churchil Angel
    Babu, Revington
    Dakhsinamurthy, Arun
    Manikandan, N.
    Palanivel, A.
    SYNTHESIS AND REACTIVITY IN INORGANIC METAL-ORGANIC AND NANO-METAL CHEMISTRY, 2016, 46 (02) : 291 - 294
  • [10] Green Synthesis of Silver Nanoparticles Using Argyreia nervosa Leaf Extract and Their Antimicrobial Activity
    Parvathalu, K.
    Chinmayee, S.
    Preethi, B.
    Swetha, A.
    Maruthi, G.
    Pritam, M.
    Sreenivas, B.
    Naidu, S. Ramu
    Merlinsheeba, G. L.
    Murali, B.
    Vijay, M.
    Moses, K.
    Krishna, D. Chinni
    Bhaskar, P. Bala
    PLASMONICS, 2023, 18 (03) : 1075 - 1081