Anti-inflammatory and antihyperalgesic effects of the combination of ibuprofen and hemin in adjuvant-induced arthritis in the Wistar rat

被引：28
作者：
Kaur S. [1 ]
Bijjem K.R.V. [1 ]
Sharma P.L. [1 ]
机构：
[1] Department of Pharmacology, ISF College of Pharmacy, Moga
来源：
Inflammopharmacology | 2011年 / 19卷 / 5期
关键词：
Adjuvant arthritis; Heme oxygenase-1; Hemin; Ibuprofen;
D O I：
10.1007/s10787-011-0090-8
中图分类号：
学科分类号：
摘要：
Aim: Although, pharmacological activation of heme oxygenase (HO)-1 has shown to produce ameliorative effects in various experimental models of inflammation, but such beneficial effects have not been observed in adjuvant-induced arthritis. Further, the upregulated activity of HO-1 has been implicated in the pathogenesis of adjuvant arthritis. The present study was designed to investigate the anti-inflammatory and antihyperalgesic effects of the prophylactic use of hemin alone and/or in combination with ibuprofen using adjuvant-induced arthritis in Wistar rat. Methods: Arthritis was induced by an intradermal injection of complete Freund's adjuvant (CFA) into left hind paw. Paw volume, thermal hyperalgesia, mechanical allodynia, joint stiffness and mobility behaviors (score) were measured. Results: Administration of ibuprofen (8.75, 17.5, 35 mg/kg/day, p.o.) and hemin (1, 5, 10 mg/kg/day, i.p.) were significantly effective in suppressing CFA-induced paw oedema, thermal and mechanical hyperalgesia, joint stiffness and mobility. The combination of low doses of ibuprofen (8.75 mg/kg, p.o.) and hemin (1 mg/kg, i.p.) significantly reduced paw volume, thermal and mechanical hyperalgesia, as compared to the individual dose of the ibuprofen and hemin alone. Conclusions: Hence, it may be concluded that the prophylactic administration of either hemin produced significantly enhanced anti-inflammatory and analgesic effects. Further, concurrent low dose administration of hemin and ibuprofen produced significantly enhanced anti-inflammatory and analgesic effects, as compared to the either treatment alone, in CFA-induced arthritis in Wistar rats. © 2011 Springer Basel AG.
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页码：265 / 272
页数：7
相关论文
共 48 条
  • [1] Abraham N.G., Kappas A., Pharmacological and clinical aspects of heme oxygenase, Pharmacological Reviews, 60, 1, pp. 79-127, (2008)
  • [2] Aburaya M., Tanaka K.-I., Hoshino T., Tsutsumi S., Suzuki K., Makise M., Akagi R., Mizushima T., Heme oxygenase-1 protects gastric mucosal cells against non-steroidal anti-inflammatory drugs, Journal of Biological Chemistry, 281, 44, pp. 33422-33432, (2006)
  • [3] Alcaraz M.J., Fernandez P., Guillen M.I., Anti-inflammatory actions of the heme oxygenase-1 pathway, Current Pharmaceutical Design, 9, 30, pp. 2541-2551, (2003)
  • [4] Anderson K.E., Collins S., Open-label study of hemin for acute porphyria: Clinical, practice implications, Am J Med, 119, 801, (2006)
  • [5] Arevalo M.I., Escribano E., Calpena A., Domenech J., Queralt J., Thermal Hyperalgesia and Light Touch Allodynia after Intradermal Mycobacterium butyricum Administration in Rat, Inflammation, 27, 5, pp. 293-299, (2003)
  • [6] Bae S.C., Kim S.J., Sung M.K., Inadequate antioxidant nutrient intake and altered plasma antioxidant status of rheumatoid arthritis patients, J Am Coll Nutr, 22, pp. 311-315, (2003)
  • [7] De Bandt M., Grossin M., Driss F., Pincemail J., Babin-Chevaye C., Pasquier C., Vitamin E uncouples joint destruction and clinical inflammation in a transgenic mouse model of rheumatoid arthritis, Arthritis and Rheumatism, 46, 2, pp. 522-532, (2002)
  • [8] Benallaoua M., Francois M., Batteux F., Thelier N., Shyy J.Y.-J., Fitting C., Tsagris L., Boczkowski J., Savouret J.-F., Corvol M.-T., Poiraudeau S., Rannou F., Pharmacologic induction of heme oxygenase 1 reduces acute inflammatory arthritis in mice, Arthritis and Rheumatism, 56, 8, pp. 2585-2594, (2007)
  • [9] Bertorelli R., Corradini L., Rafiq K., Et al., Nociceptin and the ORL-1 ligand [Phe1ψ (CH2-NH) Gly2]nociceptin(1-13) NH2 exert antiopioid effects in the Freund's adjuvant-induced arthritic rat model of chronic pain, Br J Pharmacol, 128, pp. 1252-1258, (1999)
  • [10] Bickel A., Dorfs S., Schmelz M., Forster C., Uhl W., Handwerker H.O., Effects of antihyperalgesic drugs on experimentally induced hyperalgesia in man, Pain, 76, 3, pp. 317-325, (1998)
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