A genetic linkage map construction for sesame (Sesamum indicum L.)

被引:0
作者
Li-Bin Wei
Hai-Yang Zhang
Yong-Zhan Zheng
Hong-Mei Miao
Tian-Zhen Zhang
Wang-Zhen Guo
机构
[1] Nanjing Agricultural University,National Key Laboratory of Crop Genetics & Germplasm Enhancement, Cotton Research Institute
[2] Henan Key Laboratory of Crop Improvement,undefined
来源
Genes & Genomics | 2009年 / 31卷
关键词
L.; EST-SSR; AFLP; RSAMPL; linkage map;
D O I
暂无
中图分类号
学科分类号
摘要
Sesame (Sesamum indicum L.) is one of the oldest oilseed crops with high seed oil quality. The first sesame genetic linkage map based on F2 segregating population of an intraspecific cross between two cultivars was constructed. Using three types of PCR-based markers, 284 polymorphic loci including 10 EST-SSR marker, 30 AFLP marker and 244 RSAMPL marker, respectively, had been screened. Subsequently, a total of 220 molecular markers were mapped in 30 linkage groups covering a genetic length of 936.72 cM, and the average distance between markers was 4.93 cM. In this map, the linkage groups contained from 2 to 33 loci each and ranged in distance from 6.44 cM to 74.52 cM. Based on map information, sesame genome length was estimated to be approximately 1,232.53 cM, and genome coverage of this map was about 76.0%. As a starting point of sesame genome study, the genetic linkage map will be hopeful to tag traits of breeding interest and further aid in the sesame molecular breeding. Furthermore, RSAMPL marker had been also appreciated in this paper, for its first usage in genetic map construction and higher utilization potential in some crop species lacking much genome information.
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页码:199 / 208
页数:9
相关论文
共 218 条
[1]  
Ashri A(1998)Sesame breeding Plant Breed. Review 16 179-228
[2]  
Bhat KV(1999)Study of genetic diversity in Indian and exotic sesame ( Euphytica 110 21-33
[3]  
Babrekar PP(1980) L.) germplasm using random amplified polymorphic DNA (RAPD) markers Am. J. Hum. Genet. 32 314-331
[4]  
Lakhanpaul S(2004)Construction of a genetic linkage map in man using restriction fragment length polymorphisms Theor. Appl. Genet. 108 814-821
[5]  
Bostein D(1999)An intraspecific genetic map of velvetbean ( Theor. Appl. Genet. 99 425-431
[6]  
White RL(1994).) based on AFLP markers Genetics 138 1251-1274
[7]  
Skolnick M(2004)AFLP markers in a molecular linkage map of maize: codominant scoring and linkage group distribution Genet. Res. Crop Evol. 51 599-607
[8]  
Davis RW(2001)Saturated molecular map of the rice genome based on an interspecific backcross population Genetics 159 1701-1716
[9]  
Capo-chichi LJA(1999)Analysis of genetic diversity in Turkish sesame ( Theor. Appl. Genet. 99 254-271
[10]  
Morton CM(2004) L.) populations using RAPD markers Mol. Gen. Genomics 272 308-327