Occurrence and Diagnosis of Tomato chlorosis virus in Portugal

被引：0

作者：

D. Louro

G.P. Accotto

A.M. Vaira

机构：

[1] Direcção-Geral de Protecção das Culturas –,

[2] Istituto di Fitovirologia Applicata – CNR –,undefined

[3] Istituto di Fitovirologia Applicata – CNR –,undefined

来源：

European Journal of Plant Pathology | 2000年 / 106卷

关键词：

ToCV; tomato; whiteflies;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

Tomato chlorosis virus (ToCV), a new whitefly-transmitted and phloem-limited Crinivirus infecting tomatoes in Europe, is reported for the first time in Portugal. Tomato plants with symptoms of interveinal chlorosis, collected during autumn 1998 and summer and autumn 1999 in Algarve, southern Portugal, were positive in RT-PCR assays using ToCV-specific primers. The amplified 439 bp fragment was sequenced and showed 99% homology with the ToCV sequence in the GenBank database. A digoxigenin–DNA probe was produced and tested in dot-blot with total RNAs extracted from tomato samples. Both the RT-PCR and dot-blot hybridisation procedures enabled rapid and reliable detection of ToCV from field samples.

引用

页码：589 / 592

页数：3

共 19 条

[1]

Accotto GP(2000)Typing of tomato yellow leaf curl viruses in Europe Eur J Plant Pathol 106 179-186

[2]

Navas-Castillo J(1996)Tomato infectious chlorosis virus-A new clostero-like virus transmitted by Eur J Plant Pathol 102 219-226

[3]

Noris E(1999)Abbreviations for plant virus names-1999 Arch Virol 144 1249-1273

[4]

Moriones E(1998)Ecology and epidemiology of whitefly-transmitted closteroviruses Plant Dis 82 270-279

[5]

Louro D(1998)Tomato chlorosis virus: a new whitefly-transmitted, phloemlimited, bipartite closterovirus of tomato Phytopathology 88 402-409

[6]

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[10]

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