Detection of disseminated lung cancer cells in regional lymph nodes by assay of CK19 reverse transcriptase polymerase chain reaction and its clinical significance

被引:0
作者
Ming Jian Ge
Qing Chen Wu
Mei Wang
Yu Hong Zhang
Liang Bin Li
机构
[1] Chongqing University of Medical Science,Department of Thoracic Surgery [MJ G, QC W and LB L] and Clinical Laboratory [M W and YH Z], The First Affiliated Hospital
来源
Journal of Cancer Research and Clinical Oncology | 2005年 / 131卷
关键词
Lung neoplasm; Lymphatic metastasis; Keratin; Polymerase chain reaction;
D O I
暂无
中图分类号
学科分类号
摘要
Purpose: To set up a molecular method (i.e. RT-PCR) that can be used to detect disseminated tumor cells (DTCs) in regional lymph nodes (LNs) in patients with lung cancer and to evaluate its clinical significance.
引用
收藏
页码:662 / 668
页数:6
相关论文
共 206 条
  • [1] Pelkey TJ(1996)Molecular and immunological detection of circulating tumor cells and micrometastases from solid tumor Clin Chem 42 1369-1381
  • [2] Frierson HF(1995)Prostate-specific antigen messenger RNA is expressed in non-prostate cells: implication for detection of micrometastases Cancer Res 55 2640-2644
  • [3] Bruns DE(1985)Molecular structure of the human cytoplasmic β-action gene: interspecies homology of sequences in the introns Proc Natl Acad Sci USA 82 6133-6137
  • [4] Smith MR(1988)Low level expression of cytokeratin 8, 18 and 19 in vascular smooth muscle cells of human umbilical cord and in cultured cell derived therefrom, with an analysis of the chromosomal locus containing the cytokeratin 19 gene Eur J Cell Bio 47 300-319
  • [5] Biggar S(1989)Single-step method of RNA isolation by acid guanidinium thoicyanate-phenol-chloroform extraction Anal Biochem 162 156-159
  • [6] Hussain M(1998)Interference of human processed pseudogene and corresponding countermeasures in reverse transcriptase-polymerase chain reaction Chin J Med Genet 15 170-172
  • [7] Nakajima-Iijima S(1993)Keratin gene expression in no-epithelial tissue Am J Pathol 142 1111-1118
  • [8] Hamada H(2005)Association of CK19 mRNA detection of occult cancer cells in mediastinal lymph nodes in non-small cell lung carcinoma and high risk of early recurrence. Eur J Cancer 41 306-312
  • [9] Reddy P(1996)Keratin 19 gene mRNA measurement to detect micrometastases in lymph nodes in breast cancer patients Br J Cancer 74 1639-1642
  • [10] Kakunaga T(2001)Nodal occult metastasis in patients with peripheral lung adenocarcinoma of 2.0 cm or less in diameter Ann Thorac Surg 71 1772-1778