Physical changes of amniotic membranes through glycerolization for the use as an epidermal substitute. Light and electron microscopic studies

被引：20

作者：

Rejzek A. ^{[1
]}

Weyer F. ^{[1
]}

Eichberger R. ^{[2
]}

Gebhart W. ^{[2
]}

机构：

[1] Department of Plastic and Reconstructive Surgery, General Hospital, 3100 St. Pölten

[2] Department of Dermatology, General Hospital, St. Pölten

来源：

Cell and Tissue Banking | 2001年 / 2卷 / 2期

关键词：

Amnion; Epidermal substitution; Glycerol conservation; Microscopic findings;

D O I：

10.1023/A:1014316232009

中图分类号：

学科分类号：

摘要：

Our Department of Plastic and Reconstructive Surgery has routinely been using amnion preserved in glycerol for the treatment of debrided II° burns. This treatment is almost pain free and requires fewer changes of dressings and fewer anaesthetics. It also prevents overgrowing granulation tissue and lessens scarring. Since 1910 amnion has been used as biological wound dressing. Its advantages such as reduced loss of protein and electrolytes, fluids and energy as well as reducing the risk of infection and accelerated regeneration of the epithelium have been well documented in medical literature. In order to more closely examine the question of possible changes to the micro structure of the amnion through preservation and rehydration as well as the interaction between transplanted tissue and recipient, we have carried out several light and electron microscopic studies. Results showed that neither the treatment with glycerol, nor the pretransplantation rehydration eliminates the monolayer of surface epithelium of the amnion. Its complex architecture remains intact during the preservation process and is therefore suitable as a matrix for the growth of keratinocytes and thereby the healing process. In clinical use we found amnion to be an excellent wound dressing as it allows proper control of fluid, has sufficient permeability for gases, has good thermal properties, is impervious to micro-organisms and is free from toxic material.

引用

页码：95 / 102

页数：7

共 25 条

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