In-gel digestion for mass spectrometric characterization of proteins and proteomes

被引：0
作者：
Andrej Shevchenko
Henrik Tomas
Jan Havli
Jesper V Olsen
Matthias Mann
机构：
[1] Max Planck Institute of Molecular Cell Biology and Genetics,
[2] Pfotenhauerstrasse 108,undefined
[3] Max Planck Institute for Biochemistry,undefined
[4] Am Klopferspitz 18,undefined
来源：
Nature Protocols | 2006年 / 1卷
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摘要：
In-gel digestion of proteins isolated by gel electrophoresis is a cornerstone of mass spectrometry (MS)-driven proteomics. The 10-year-old recipe by Shevchenko et al. has been optimized to increase the speed and sensitivity of analysis. The protocol is for the in-gel digestion of both silver and Coomassie-stained protein spots or bands and can be followed by MALDI-MS or LC-MS/MS analysis to identify proteins at sensitivities better than a few femtomoles of protein starting material.
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页码：2856 / 2860
页数：4
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