Molecular characterization of Hop latent virus and phylogenetic relationships among viruses closely related to carlaviruses

被引：0

作者：

T. Hataya

K. Uchino

R. Arimoto

N. Suda

T. Sano

E. Shikata

I. Uyeda

机构：

[1] Laboratory of Pathogen-Plant Interactions,

[2] Plant Breeding Science,undefined

[3] Graduate School of Agriculture,undefined

[4] Hokkaido University,undefined

[5] Sapporo,undefined

[6] Hokkaido,undefined

[7] Japan,undefined

[8] Hop Research Department,undefined

[9] Plant Bioengineering Research Laboratories,undefined

[10] Sapporo Breweries Ltd.,undefined

[11] Hokkaido,undefined

[12] Japan,undefined

[13] Department of Environmental Biology,undefined

[14] Faculty of Agriculture and Life Science,undefined

[15] Hirosaki University,undefined

[16] Aomori,undefined

[17] Japan,undefined

来源：

Archives of Virology | 2000年 / 145卷

关键词：

Peptide; Phylogenetic Relationship; Coat Protein; Virus Particle; Molecular Characterization;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

The complete nucleotide sequence of the hop latent virus (HpLV) genome was determined. The viral RNA genome is 8,612 nucleotides long, excluding the poly(A) tail, and contains six open reading frames (ORFs), which encode putative proteins of 224-kDa (ORF 1), 25-kDa (ORF 2), 11-kDa (ORF 3), 7-kDa (ORF 4), 34-kDa (ORF 5), and 12-kDa (ORF 6). ORF 5 encodes the coat protein as demonstrated by N-terminal sequencing of three proteolytic peptides derived from the virus particle. The genome organization of HpLV is similar to that of other species in the genus Carlavirus, and the overall sequence of HpLV is more similar to that of Potato virus M than to sequences of other carlaviruses reported to date. The amino acid sequences of the putative methyltransferase, RNA helicase, and RNA-dependent RNA polymerase encoded in ORF 1 and an ‘accessory’ helicase encoded in ORF 2 of the HpLV genome were compared with those of viruses in the ‘tymo’ lineage: the genera Carlavirus, Potexvirus, Allexivirus, Foveavirus, Trichovirus, Capillovirus, Vitivirus, and Tymovirus. The phylogenetic relationships among the viruses in these genera are discussed. This is the first molecular characterization of a carlavirus infecting hop plants.

引用

页码：2503 / 2524

页数：21