Resonance Double Light Scattering Method for the Determination of Nucleic Acids with Cetylpyridine Bromide

被引:5
|
作者
Rutao Liu
Jinghe Yang
Changxia Sun
Xia Wu
Xibao Gao
Yang Liu
Shufang Liu
Benyu Su
机构
[1] Key Laboratory of Colloid and Interface Chemistry,
[2] Ministry of Education,undefined
[3] School of Chemistry and Chemical Engineering,undefined
[4] Shandong University,undefined
[5] Shandong Supervision and Inspection Institute for Product Quality,undefined
来源
Microchimica Acta | 2004年 / 147卷
关键词
Key words: Resonance double light scattering; nucleic acids.;
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学科分类号
摘要
Cetylpyridine bromide (CPB) was used as a novel probe to determine nucleic acids by the resonance double light scattering technique in this paper. Under the optimum conditions, different nucleic acids have different binding properties with CPB. The sensitivity of this method decreases in the following order: ctDNA>yRNA>fsDNA. The detection limits are 8.9, 12.7 and 18.7 ng mL−1, respectively. Synthetic samples were analyzed satisfactorily.
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页码:105 / 109
页数:4
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