Aberrant Methylation of the SOX21-AS1 Promoter Region Promotes Gene Expression and Its Clinical Value in Cervical Cancer

被引：0
作者：
Peipei Du
Yanfang Zhi
Ruijie Wang
Ya Li
Huanhuan Li
Xiaoan Zhang
Guomei Cheng
Xiaofu Li
机构：
[1] The Third Affiliated Hospital of Zhengzhou University,Department of Cytopathology
[2] Academy of Medical Sciences of Zhengzhou University,Department of Cytopathology
[3] The Third Affiliated Hospital of Zhengzhou University,Department of Obstetrics and Gynecology
[4] The Third Affiliated Hospital of Zhengzhou University,Department of Imaging
来源：
Reproductive Sciences | 2021年 / 28卷
关键词：
Long non-coding RNA; Promoter methylation; SOX21-AS1; Cervical cancer; Diagnosis;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Cervical cancer is the fourth most common female cancer worldwide. Long non-coding RNAs (lncRNAs), such as SOX21-AS1, play pivotal roles in the progression and metastasis of cancer. We previously described that SOX21-AS1 was hypomethylated in cervical cancer (CC) and aimed to further explore the relationship between methylation of the SOX21-AS1 promoter and CC using clinical cervical samples. Pyrosequencing was performed to detect the methylation status of the SOX21-AS1 promoter in 33 cervical specimens. Additionally, expression levels of related genes in 43 clinical cervical specimens were measured using quantitative real-time PCR (qRT-PCR). The SOX21-AS1 promoter was significantly hypomethylated in CC (P < 0.01). SOX21-AS1 hypomethylation was also significantly associated with an advanced Federation of Gynecology and Obstetrics (FIGO) stage (P < 0.01). The expression levels of SOX21-AS1 and SOX21 were noted to be higher in cancer vs. normal cervix (all P < 0.001). Moreover, the expression of SOX21-AS1 was positively correlated with SOX21 in all samples (r = 0.891, P < 0.001). Methylation statue of the SOX21-AS1 promoter region was negatively correlated with the expression levels of SOX21-AS1 and SOX21 (SOX21-AS1, r = − 0.628; SOX21, r = − 0.648; both P < 0.001). The methylation status of SOX21-AS1 displayed promising diagnostic potential for CC, exhibiting good sensitivity (100.0%) and specificity (69.2%), with an area under the curve of 0.846. In addition, bioinformatic analyses identified a potential link between SOX21-AS1 and the Wnt signaling pathway. Furthermore, methylation status of SOX21-AS1 was negatively correlated with β-catenin/c-myc/cyclin D1 mRNA levels (rs = − 0.529, − 0.462 ,and − 0.383, respectively, P < 0.05). Our findings illuminated that lncRNA SOX21-AS1 showed hypomethylation in cervical cancer and SOX21-AS1 could serve as a novel biomarker for CC diagnosis or a potential therapeutic target.
引用
收藏
页码：532 / 540
页数：8
相关论文
共 50 条
[41]   DNA methylation promotes the expression of PPARy transcript 1 at least in part by preventing NRF1 binding to the promoter P1 of chicken PPARy gene [J].
Cui, T. T. ;
Huang, J. X. ;
Ning, B. L. ;
Mu, F. ;
Chen, H. Y. ;
Xing, T. Y. ;
Li, H. ;
Wang, N. .
POULTRY SCIENCE, 2024, 103 (05)
[42]   Aberrant Expression of miR-142-3p and its Target Gene HMGA1 and FZD7 in Breast Cancer and its Clinical Significance [J].
Jia, Xiu-Peng ;
Meng, Ling-Li ;
Fang, Jian-Chen ;
Wang, Hong-Wei ;
Chen, Jie ;
Zhou, Jue ;
Wang, Chun-Nian ;
Jiang, Wei-Feng .
CLINICAL LABORATORY, 2018, 64 (06) :915-921
[43]   The application value of PAX1 and ZNF582 gene methylation in high grade intraepithelial lesion and cervical cancer [J].
H. Liang ;
G. L. Li ;
J. Liu ;
M. Fu ;
H. Huang ;
K. Zhao ;
Y. Wei ;
J. Xiao .
Clinical and Translational Oncology, 2021, 23 :283-288
[44]   The application value of PAX1 and ZNF582 gene methylation in high grade intraepithelial lesion and cervical cancer [J].
Liang, H. ;
Li, G. L. ;
Liu, J. ;
Fu, M. ;
Huang, H. ;
Zhao, K. ;
Wei, Y. ;
Xiao, J. .
CLINICAL & TRANSLATIONAL ONCOLOGY, 2021, 23 (02) :283-288
[45]   Decreased expression of angiogenesis antagonist EFEMP1 in sporadic breast cancer is caused by aberrant promoter methylation and points to an impact of EFEMP1 as molecular biomarker [J].
Sadr-Nabavi, Ariane ;
Ramser, Juliane ;
Volkmann, Juliane ;
Naehrig, Joerg ;
Wiesmann, Frank ;
Betz, Beate ;
Hellebrand, Heide ;
Engert, Stefanie ;
Seitz, Susanne ;
Kreutzfeld, Rene ;
Sasaki, Takako ;
Arnold, Norbert ;
Schmutzler, Rita ;
Kiechle, Marion ;
Niederacher, Dieter ;
Harbeck, Nadia ;
Dahl, Edgar ;
Meindl, Alfons .
INTERNATIONAL JOURNAL OF CANCER, 2009, 124 (07) :1727-1735
[46]   Evaluation of glutathione S-transferase pi 1 expression and gene promoter methylation in Moroccan patients with urothelial bladder cancer [J].
Hadami, Khaoula ;
Dakka, Nadia ;
Bensaid, Mounia ;
El Ahanidi, Hajar ;
Ameur, Ahmed ;
Chahdi, Hafsa ;
Oukabli, Mohamed ;
Al Bouzidi, Abderrahmane ;
Attaleb, Mohammed ;
El Mzibri, Mohammed .
MOLECULAR GENETICS & GENOMIC MEDICINE, 2018, 6 (05) :819-827
[47]   PAX1/SOX1 gene methylation as a detection and triage method for triage of high risk HPV-Positive women in cervical cancer screening [J].
Zhang, Ruyi ;
Li, Ye ;
Han, Yu ;
Guo, Chang ;
Guo, Jie ;
Sun, Jie ;
Wang, Dengfeng ;
Hu, Xiatao ;
Li, Jiao ;
Zhao, Xuanying ;
Zhou, Yiyuan ;
Zhai, Tingting ;
Meng, Yuqiong ;
Wang, Jing ;
Chen, Wengao .
GYNECOLOGIC ONCOLOGY REPORTS, 2025, 60
[48]   FOXO1 Gene Downregulation and Promoter Methylation Exhibits Significant Correlation With Clinical Parameters in Indian Breast Cancer Patients [J].
Khan, Mohammad Aasif ;
Massey, Sheersh ;
Ahmad, Irfan ;
Akhter, Naseem ;
Habib, Maria ;
Mustafa, Saad ;
Deo, S. V. S. ;
Husain, Syed Akhtar ;
Sadaf .
FRONTIERS IN GENETICS, 2022, 13
[49]   Expression of the HPV18E2 Gene in Cervical Cancer and Premalignant Lesions and its Clinical Significance [J].
Ling Mou ;
Bin Wu ;
Ya Hu ;
Ying Lan ;
Feng-Lin Lv .
Cell Biochemistry and Biophysics, 2014, 70 :841-844
[50]   Expression of the HPV18E2 Gene in Cervical Cancer and Premalignant Lesions and its Clinical Significance [J].
Mou, Ling ;
Wu, Bin ;
Hu, Ya ;
Lan, Ying ;
Lv, Feng-Lin .
CELL BIOCHEMISTRY AND BIOPHYSICS, 2014, 70 (02) :841-844
12345