Development and Validation of an Analytical Method for the Determination of Aloin Using Liquid Chromatography Tandem Mass Spectrometry

A new simple analytical method was developed for the identification and quantification of aloin in aloe gel. The extraction was performed using acetonitrile acidified with formic acid (1%, v/v) with no additional cleanup step. The compound was determined using high-performance liquid chromatography coupled with triple quadrupole mass spectrometry (QqQ). The recovery rates obtained were between 80 and 110% with a relative standard deviation < 26%, at four different concentration levels. The calculated limit of quantification (LOQ) was at 0.05 mg kg−1.