Fabrication of transplantable human oral mucosal epithelial cell sheets using temperature-responsive culture inserts without feeder layer cells

被引：57

作者：

Murakami D. ^{[1
,2
]}

Yamato M. ^{[2
]}

Nishida K. ^{[3
]}

Ohki T. ^{[2
,4
]}

Takagi R. ^{[2
]}

Yang J. ^{[2
]}

Namiki H. ^{[1
]}

Okano T. ^{[2
]}

机构：

[1] Graduate School of Science and Engineering, Waseda University, Tokyo

[2] Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University, Shinjuku-ku, Tokyo 162-8666

[3] Department of Ophthalmology and Visual Science, Tohoku University, Graduate School of Medicine, Sendai

[4] Department of Surgery, Institute of Gastroenterology, Tokyo Women's Medical University, Tokyo

来源：

Journal of Artificial Organs | 2006年 / 9卷 / 3期

关键词：

Cell culture; Epithelial cell; Stem cell; Thermally responsive material;

D O I：

10.1007/s10047-006-0342-3

中图分类号：

学科分类号：

摘要：

To exclude bacteria- or animal-derived factors from cultured fabrication of transplantable epithelial cell sheets, primary human oral mucosal epithelial cells were seeded on temperature-responsive culture inserts having submicron-scale pores. Supplying culture medium containing human autologous serum to both apical and basal sides of human epithelial cells allows these cells to grow to confluence. These proliferating cells created stratified epithelial layers even when 3T3 feeder layers and fetal bovine serum were eliminated from culture. Normal keratin expression profiles were obtained with these cells, and basal and midlayer cells expressed p63, a putative stem/progenitor marker. These results suggest that temperature-responsive culture inserts can be useful in clinical settings that require the exclusion of xenogeneic factors. © 2006 The Japanese Society for Artificial Organs.

引用

页码：185 / 191

页数：6

共 33 条

[1]

Gallico III G.G., O'Connor N.E., Compton C.C., Kehinde O., Green H., Permanent coverage of large burn wounds with autologous cultured human epithelium, N Engl J Med, 311, pp. 448-451, (1984)

[2]

Phillips T.J., Kehinde O., Green H., Gilchrest B.A., Treatment of skin ulcers with cultured epidermal allografts, J Am Acad Dermatol, 21, pp. 191-199, (1989)

[3]

Gallico III G.G., O'Connor N.E., Compton C.C., Remensnyder J.P., Kehinde O., Green H., Cultured epithelial autografts for giant congenital nevi, Plast Reconstr Surg, 84, pp. 1-9, (1989)

[4]

Ueda M., Hata K., Horie K., Torii S., The potential of oral mucosal cells for cultured epithelium: A preliminary report, Ann Plast Surg, 35, pp. 498-504, (1995)

[5]

Hata K., Ueda M., Fabrication of cultured epithelium using oral mucosal cells and its clinical applications, Hum Cell, 9, pp. 91-96, (1996)

[6]

Tsai C.Y., Ueda M., Hata K., Horie K., Hibino Y., Sugimura Y., Toriyama K., Torii S., Clinical results of cultured epithelial cell grafting in the oral and maxillofacial region, J Craniomaxillofac Surg, 25, pp. 4-8, (1997)

[7]

Nishida K., Yamato M., Hayashida Y., Watanabe K., Yamamoto K., Adachi E., Nagai S., Kikuchi A., Maeda N., Watanabe H., Okano T., Tano Y., Corneal reconstruction with tissue-engineered cell sheets composed of autologous oral mucosal epithelium, N Engl J Med, 351, pp. 1187-1196, (2004)

[8]

Hayashida Y., Nishida K., Yamato M., Watanabe K., Maeda N., Watanabe H., Kikuchi A., Okano T., Tano Y., Ocular surface reconstruction using autologous rabbit oral mucosal epithelial sheets fabricated ex vivo on a temperature-responsive culture surface, Invest Ophthalmol Vis Sci, 46, pp. 1632-1639, (2005)

[9]

Rheinwald J.G., Green H., Serial cultivation of strains of human epidermal keratinocytes: The formation of keratinizing colonies from single cells, Cell, 6, pp. 331-343, (1975)

[10]

Green H., Kehinde O., Thomas J., Growth of cultured human epidermal cells into multiple epithelia suitable for grafting, Proc Natl Acad Sci USA, 76, pp. 5665-5668, (1979)

← 1 2 3 4 →