Construction of a lncRNA-associated competing endogenous RNA regulatory network after traumatic brain injury in mouse (vol 15, 40, 2022)

被引：0

作者：

Wang, Siqi ^{[1
]}

Sun, Yiyu ^{[2
]}

Hu, Shaobo ^{[3
]}

Lou, Cen ^{[1
]}

Pan, Yuan-Bo ^{[4
]}

机构：

[1] Zhejiang Univ, Sch Med, Sir Run Run Shaw Hosp, Dept Nucl Med, Hangzhou 310000, Zhejiang, Peoples R China

[2] Shanghai Jiao Tong Univ, Shanghai Peoples Hosp 9, Dept Plast & Reconstruct Surg, 639 Zhi Zao Ju Rd, Shanghai 200011, Peoples R China

[3] Li Hui Li Hosp, Med Ctr Ningbo, Dept Neurosurg, Ningbo, Zhejiang, Peoples R China

[4] Zhejiang Univ, Sch Med, Affiliated Hosp 2, Dept Neurosurg, 88 Jiefang Rd, Hangzhou 310009, Zhejiang, Peoples R China

来源：

MOLECULAR BRAIN | 2022年 / 15卷 / 01期

关键词：

Bioinformatics analysis; ceRNA; lncRNA; Myd88; Neat1; Traumatic brain injury;

D O I：

10.1186/s13041-022-00950-7

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Traumatic brain injury (TBI) is a major public health problem worldwide which causes high mortality and disability. Functioning as microRNA (miRNA) sponges, long non-coding RNA (lncRNA) regulates the expression of protein-coding genes in a competing endogenous RNA (ceRNA) network. However, the lncRNA-associated ceRNA in TBI remains unclear. In this study, we processed the raw SRR files of mice cortex samples of sham injury (n = 3) and TBI groups (n = 3) to count files. Then, the expression profiles of lncRNAs and mRNAs were identified, and 86 differentially expressed (DE) lncRNAs and 1201 DEmRNAs between sham and TBI groups were identified. The DEmRNAs were used to perform enrichment analyses. Next, a lncRNA-miRNA-mRNA regulatory ceRNA network was constructed. The network consisted of 23 mRNAs, 5 miRNAs and 2 lncRNAs. The expression alternations of the 5 miRNAs were validated via qRT-PCR. The subnetwork of hub lncRNA Neat1 was extracted. We identified a potential inflammatory associated regulatory axis: Neat1/miR-31-5p/Myd88 axis. The PPI network based on DEmRNA involved in ceRNA network was constructed PPI networks to identify the hub genes. Finally, DElncRNAs and DEmRNAs were selected randomly and validated by qRT-PCR. In conclusion, with the lncRNA-miRNA-mRNA ceRNA network provided above, we can improve our understanding of the regulatory mechanisms and interaction among lncRNAs, miRNAs and mRNAs in TBI process. © 2022, The Author(s).

引用

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