Tetranucleotide microsatellite loci from the endangered green turtle (Chelonia mydas)

被引:0
作者
Brian M. Shamblin
Bonnie E. Berry
Denise M. Lennon
Dean A. Bagley
Llewellyn M. Ehrhart
Campbell J. Nairn
机构
[1] University of Georgia,Daniel B. Warnell School of Forestry and Natural Resources
[2] University of Central Florida,Department of Biology
来源
Conservation Genetics Resources | 2012年 / 4卷
关键词
Green turtle; Microsatellite; Population connectivity;
D O I
暂无
中图分类号
学科分类号
摘要
We describe isolation and characterization of 20 polymorphic tetranucleotide loci from the green turtle (Chelonia mydas). We identified an average of 12.5 alleles per locus based on screening of 31 individuals foraging in the Indian River Lagoon, Florida, USA. Observed heterozygosity ranged from 0.53 to 1.00, with a mean of 0.85. This microsatellite suite has a combined non-exclusion probability of identity of 2.45 × 10–28. These markers will complement those already available for green turtles in individual-based as well as population level genetic analyses.
引用
收藏
页码:783 / 785
页数:2
相关论文
共 49 条
[1]  
Bowen BW(2007)Population genetics and phylogeography of sea turtles Mol Ecol 16 4886-4907
[2]  
Karl SA(2008)Encouraging outlook for recovery of a once severely exploited marine megaherbivore Glob Ecol Biogeogr 17 297-304
[3]  
Chaloupka M(1991)‘Touchdown’ PCR to circumvent spurious priming during gene amplification Nucleic Acids Res 19 4008-356
[4]  
Bjorndal K(2009)Characterization of polymorphic microsatellite markers from the green turtle ( Mol Ecol Resour 9 354-970
[5]  
Balazas G(2006)) Mol Ecol Notes 6 968-94
[6]  
Bolten A(2008)GMCONVERT: file conversion for GENEMAPPER output files Mol Ecol Resour 8 92-440
[7]  
Ehrhart L(1995)MSTACOMMANDER: detection of microsatellite repeat arrays and automated, locus-specific primer design Mol Biol Evol 12 432-S32
[8]  
Limpus C(1997)Conservation and dynamics of microsatellite loci over 300 million years of marine turtle evolution Coral Reefs 16 S23-1106
[9]  
Suganuma H(2007)Reefs since Columbus Mol Ecol 16 1099-296
[10]  
Troëng S(2006)Revising how the computer program CERVUS accommodates genotyping error increases success in paternity assignment Front Ecol Environ 4 290-225