High-level expression, purification and characterization of a recombinant medium-temperature α-amylase from Bacillus subtilis

被引：0

作者：

Yihan Liu

Fuping Lu

Guanqun Chen

Crystal L. Snyder

Jing Sun

Yu Li

Jianling Wang

Jing Xiao

机构：

[1] Ministry of Education,Key Laboratory of Industrial Microbiology

[2] Tianjin University of Science and Technology,College of Biotechnology

[3] University of Alberta,Department of Agricultural, Food and Nutritional Science

来源：

Biotechnology Letters | 2010年 / 32卷

关键词：

Medium-temperature alpha amylase; Over-expression; Purification;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

Alpha-amylases are important industrial enzymes with a wide range of applications. Although medium-temperature alpha amylase (AmyE) has some practical advantages, its low yield has limited its applications. When an amyE gene from Bacillus subtilis BF768 was cloned into vector pWB980 and over-expressed in B. subtilis WB600, high activities (723 U ml−1) of secreted AmyE were produced. Recombinant AmyE was purified to a specific activity of 36 U mg−1 having optimal activity at pH 6.0 and 60°C.

引用

页码：119 / 124

页数：5

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