Functionalized graphene grids with various charges for single-particle cryo-EM

被引：0

作者：

Ye Lu

Nan Liu

Yongbo Liu

Liming Zheng

Junhao Yang

Jia Wang

Xia Jia

Qinru Zi

Hailin Peng

Yu Rao

Hong-Wei Wang

机构：

[1] Tsinghua University,School of Life Sciences

[2] Tsinghua University,Tsinghua

[3] Tsinghua University,Peking Joint Center for Life Sciences

[4] Tsinghua University,Beijing Frontier Research Center for Biological Structures, Beijing Advanced Innovation Center for Structural Biology

[5] Tsinghua University,Ministry of Education Key Laboratory of Protein Sciences

[6] Tsinghua University,Ministry of Education Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology

[7] Peking University,School of Pharmaceutical Sciences

[8] Beijing Graphene Institute,Center for Nanochemistry, Beijing Science and Engineering Center for Nanocarbons, Beijing National Laboratory for Molecular Sciences, College of Chemistry and Molecular Engineering

[9] Wuhan University,College of Life Sciences

来源：

Nature Communications | / 13卷

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摘要：

A major hurdle for single particle cryo-EM in structural determination lies in the specimen preparation impaired by the air-water interface (AWI) and preferential particle-orientation problems. In this work, we develop functionalized graphene grids with various charges via a dediazoniation reaction for cryo-EM specimen preparation. The graphene grids are paraffin-assistant fabricated, which appear with less contaminations compared with those produced by polymer transfer method. By applying onto three different types of macromolecules, we demonstrate that the high-yield charged graphene grids bring macromolecules away from the AWI and enable adjustable particle-orientation distribution for more robust single particle cryo-EM structural determination.

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