Functionalized graphene grids with various charges for single-particle cryo-EM

被引：0
|
作者：
Ye Lu
Nan Liu
Yongbo Liu
Liming Zheng
Junhao Yang
Jia Wang
Xia Jia
Qinru Zi
Hailin Peng
Yu Rao
Hong-Wei Wang
机构：
[1] Tsinghua University,School of Life Sciences
[2] Tsinghua University,Tsinghua
[3] Tsinghua University,Peking Joint Center for Life Sciences
[4] Tsinghua University,Beijing Frontier Research Center for Biological Structures, Beijing Advanced Innovation Center for Structural Biology
[5] Tsinghua University,Ministry of Education Key Laboratory of Protein Sciences
[6] Tsinghua University,Ministry of Education Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology
[7] Peking University,School of Pharmaceutical Sciences
[8] Beijing Graphene Institute,Center for Nanochemistry, Beijing Science and Engineering Center for Nanocarbons, Beijing National Laboratory for Molecular Sciences, College of Chemistry and Molecular Engineering
[9] Wuhan University,College of Life Sciences
来源：
Nature Communications | / 13卷
关键词：
D O I：
暂无
中图分类号：
学科分类号：
摘要：
A major hurdle for single particle cryo-EM in structural determination lies in the specimen preparation impaired by the air-water interface (AWI) and preferential particle-orientation problems. In this work, we develop functionalized graphene grids with various charges via a dediazoniation reaction for cryo-EM specimen preparation. The graphene grids are paraffin-assistant fabricated, which appear with less contaminations compared with those produced by polymer transfer method. By applying onto three different types of macromolecules, we demonstrate that the high-yield charged graphene grids bring macromolecules away from the AWI and enable adjustable particle-orientation distribution for more robust single particle cryo-EM structural determination.
引用
收藏
相关论文
共 50 条
  • [1] Functionalized graphene grids with various charges for single-particle cryo-EM
    Lu, Ye
    Liu, Nan
    Liu, Yongbo
    Zheng, Liming
    Yang, Junhao
    Wang, Jia
    Jia, Xia
    Zi, Qinru
    Peng, Hailin
    Rao, Yu
    Wang, Hong-Wei
    NATURE COMMUNICATIONS, 2022, 13 (01)
  • [2] Single-particle cryo-EM analysis of the purinosome
    Calise, S. J.
    Molfino, J.
    Dickinson, M. S.
    Quispe, J.
    Kollman, J. M.
    MOLECULAR BIOLOGY OF THE CELL, 2023, 34 (02) : 675 - 676
  • [3] A potential difference for single-particle cryo-EM
    Rosenthal, Peter B.
    IUCRJ, 2019, 6 : 988 - 989
  • [4] Single-particle cryo-EM: beyond the resolution
    Jean-Paul Armache
    Yifan Cheng
    NationalScienceReview, 2019, 6 (05) : 864 - 866
  • [5] Single-particle cryo-EM at atomic resolution
    Nakane, Takanori
    Kotecha, Abhay
    Sente, Andrija
    McMullan, Greg
    Masiulis, Simonas
    Brown, Patricia M. G. E.
    Grigoras, Ioana T.
    Malinauskaite, Lina
    Malinauskas, Tomas
    Miehling, Jonas
    Uchanski, Tomasz
    Yu, Lingbo
    Karia, Dimple
    Pechnikova, Evgeniya V.
    de Jong, Erwin
    Keizer, Jeroen
    Bischoff, Maarten
    McCormack, Jamie
    Tiemeijer, Peter
    Hardwick, Steven W.
    Chirgadze, Dimitri Y.
    Murshudov, Garib
    Aricescu, A. Radu
    Scheres, Sjors H. W.
    NATURE, 2020, 587 (7832) : 152 - +
  • [6] Single-particle cryo-EM at atomic resolution
    Sente, A.
    Nakane, T.
    Kotecha, A.
    Aricescu, A. R.
    Scheres, S. H. W.
    ACTA CRYSTALLOGRAPHICA A-FOUNDATION AND ADVANCES, 2020, 76 : A220 - A220
  • [7] Single-Particle Cryo-EM at Crystallographic Resolution
    Cheng, Yifan
    CELL, 2015, 161 (03) : 450 - 457
  • [8] Single-particle cryo-EM at atomic resolution
    Takanori Nakane
    Abhay Kotecha
    Andrija Sente
    Greg McMullan
    Simonas Masiulis
    Patricia M. G. E. Brown
    Ioana T. Grigoras
    Lina Malinauskaite
    Tomas Malinauskas
    Jonas Miehling
    Tomasz Uchański
    Lingbo Yu
    Dimple Karia
    Evgeniya V. Pechnikova
    Erwin de Jong
    Jeroen Keizer
    Maarten Bischoff
    Jamie McCormack
    Peter Tiemeijer
    Steven W. Hardwick
    Dimitri Y. Chirgadze
    Garib Murshudov
    A. Radu Aricescu
    Sjors H. W. Scheres
    Nature, 2020, 587 : 152 - 156
  • [9] Single-particle cryo-EM: beyond the resolution
    Armache, Jean-Paul
    Cheng, Yifan
    NATIONAL SCIENCE REVIEW, 2019, 6 (05) : 864 - 866
  • [10] Cryo-EM and Single-Particle Analysis with Scipion
    Jimenez-Moreno, A.
    del Cano, L.
    Martinez, M.
    Ramirez-Aportela, E.
    Cuervo, A.
    Melero, R.
    Sanchez-Garcia, R.
    Strelak, D.
    Fernandez-Gimenez, E.
    de Isidro-Gomez, F. P.
    Herreros, D.
    Conesa, P.
    Fonseca, Y.
    Maluenda, D.
    Jimenez de la Morena, J.
    Macias, J. R.
    Losana, P.
    Marabini, R.
    Carazo, J. M.
    Sorzano, C. O. S.
    JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, 2021, (171):
12345