Tryptophan depletion during continuous CSF sampling in healthy human subjects

被引：0

作者：

Carpenter L.L. ^{[1
]}

Anderson G.M. ^{[2
]}

Pelton G.H. ^{[3
]}

Gudin J.A. ^{[4
]}

Kirwin P.D.S. ^{[3
]}

Price L.H. ^{[1
]}

Heninger G.R. ^{[3
]}

McDougle C.J. ^{[5
]}

机构：

[1] Butler Hospital, Lab. Clin. Neurosci., Dept. P., Providence, RI

[2] Yale Child Study Center, Yale University School of Medicine, New Haven, CT

[3] Clinical Neuroscience Research Unit, Connecticut Mental Hlth. Ctr., D., New Haven, CT

[4] Department of Anesthesia, Yale University School of Medicine, New Haven, CT

[5] Child and Adolescent Psychiatry, Indiana Univ. School of Medicine, Indianapolis, IN

来源：

Neuropsychopharmacology | 1998年 / 19卷 / 1期

关键词：

5-HIAA; Amino acids; CSF; Serotonin; Tryptophan;

D O I：

10.1016/S0893-133X(97)00198-X

中图分类号：

学科分类号：

摘要：

The tryptophan (TRP) depletion paradigm has been employed to investigate mood and behavioral effects of acutely lowering plasma TRP, and presumably brain serotonin (5-hydroxytryptamine [5-HT]) levels through administration of a special diet and/or amino acid drink. Our goal was to test the assumption that a corresponding fall in central levels of TRP and 5-HT (measured by its major metabolite, 5-hydroxyindoleacetic acid [5-HIAA]) occurs during the standard execution of this method in healthy adult subjects. Three males and two females completed the protocol, which included a one-day low-TRP diet and a TRP-free amino acid drink. Lumbar puncture was performed, with placement of an indwelling catheter connected to a peristaltic pump and fraction collector. Cerebrospinal fluid (CSF) was sampled continuously for a 13.5- hour period (before, during, and after the drink) with fractions removed every 15 minutes. Plasma samples were simultaneously obtained. CSF TRP levels and plasma TRP levels were highly correlated, falling a mean of 92% and 85% from baseline, respectively. CSF nadirs were reached several hours after plasma nadirs. CSF 5-HIAA decreased modestly (24% to 40%, mean 31% change from baseline), with lowest concentrations observed 8-12 hours after the amino acid drink. These data suggest that TRP depletion results in substantial declines in central 5-HT turnover.

引用

页码：26 / 35

页数：9

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