Somatic stability of microsatellite loci in Eastern white pine, Pinus strobus L.

被引：0
作者：
D Cloutier
D Rioux
J Beaulieu
D J Schoen
机构：
[1] McGill University,Department of Biology
[2] Canadian Forest Service,undefined
[3] Laurentian Forestry Centre,undefined
[4] Sainte-Foy,undefined
来源：
Heredity | 2003年 / 90卷
关键词：
trees; marker loci; somatic mutation;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Variation at nuclear- and chloroplast-encoded microsatellite loci was studied among and within clonally propagated individuals of Eastern white pine. Total DNA was extracted and assayed from gamete-bearing tissue (megagametophytes) located on six different branch positions on each of 12 individual genets. No within-individual variation was observed among 12 loci studied. Estimates of numbers of mitotic cell divisions required to produce the tissue used as the source of genomic DNA were obtained by combining tree growth and anatomical data. This allowed for the calculation of upper bound estimates of numbers of mutations per locus per somatic cell division. The estimated somatic mutation rate was found to be substantially lower than those published for genomic microsatellite mutation rates in other plant species.
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页码：247 / 252
页数：5
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