Photobiomodulation reduces oxidative stress in diabetic wounded fibroblast cells by inhibiting the FOXO1 signaling pathway

被引:0
作者
Naresh Kumar Rajendran
Nicolette Nadene Houreld
Heidi Abrahamse
机构
[1] University of Johannesburg,Laser Research Centre, Faculty of Health Sciences
来源
Journal of Cell Communication and Signaling | 2021年 / 15卷
关键词
Photobiomodulation; PBM; FOXO1; AKT; Antioxidants; Oxidative Stress;
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摘要
This study aimed to elucidate the underlying molecular mechanism of photobiomodulation (PBM) in attenuating oxidative stress in diabetic wounded fibroblast cells. Cell models were exposed to PBM at a wavelength of 660 nm (fluence of 5 J/cm2, and power density of 11.2 mW/cm2) or 830 nm (fluence of 5 J/cm2, and power density of 10.3 mW/cm2). Non-irradiated cell models were used as controls. Cellular migration was determined at regular time intervals (0, 12, 24 and 48 h) using inverted light microscopy. Cell viability was determined by the Trypan blue exclusion assay. The levels of enzymic antioxidants superoxide dismutase (SOD), catalase (CAT), and heme oxygenase (HMOX1) were determined by the enzyme linked immunosorbent assay (ELISA). The alteration in the levels of AKT and FOXO1 was determined by immunofluorescence and western blotting. Upon PBM treatment, elevated oxidative stress was reversed in diabetic and diabetic wounded fibroblast cells. The reduced oxidative stress was represented by decreased FOXO1 levels and increased levels of SOD, CAT and HMOX1. This might be due to the activation of the AKT signaling pathway. This study concluded that treatment with PBM progressed diabetic wound healing by attenuating oxidative stress through inhibition of the FOXO1 signaling pathway.
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页码:195 / 206
页数:11
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  • [1] Ahmed OM(2018)Quercetin and low level laser therapy promote wound healing process in diabetic rats via structural reorganization and modulatory effects on inflammation and oxidative stress Biomed Pharmacother 101 58-73
  • [2] Mohamed T(2015)Low-level light/laser therapy versus photobiomodulation therapy Photomed Laser Surg 33 183-184
  • [3] Moustafa H(2013)Low-level laser (light) therapy (LLLT) in skin: stimulating, healing, restoring Semin Cutan Med Surg 32 41-52
  • [4] Hamdy E(2012)Collagen production in diabetic wounded fibroblasts in response to low-intensity laser irradiation at 660 nm Diabetes Technol Ther 14 1110-1117
  • [5] Anders JJ(2017)Photobiomodulation alters matrix protein activity in stressed fibroblast cells in vitro J Biopho 11 e201700127-338
  • [6] Lanzafame RJ(2011)Effect of selenium on connexin expression, angiogenesis, and antioxidant status in diabetic wound healing Biol Trace Elem Res 144 327-1837
  • [7] Arany PR(2005)Inhibition of superoxide generation and associated nitrosative damage is involved in metallothionein prevention of diabetic cardiomyopathy Diabetes 54 1829-835
  • [8] Avci P(2013)Metallothionein prevents diabetes-induced cardiac pathological changes, likely via the inhibition of succinyl-CoA:3-ketoacid coenzyme A transferase-1 nitration at Trp(374) Am J Physiol Endocrinol Metab 304 826-62
  • [9] Gupta A(2014)Biological effects of low level laser therapy Lasers Med Sci 5 58-129
  • [10] Magesh S(2015)Mallotus philippinensis Muell. Arg fruit glandular hairs extract promotes wound healing on different wound model in rats BMC Comp Alt Med 15 123-978