Solid-state NMR 13C and 15N resonance assignments of a seven-transmembrane helical protein Anabaena Sensory Rhodopsin

被引：0

作者：

Shenlin Wang

Lichi Shi

Takashi Okitsu

Akimori Wada

Leonid S. Brown

Vladimir Ladizhansky

机构：

[1] University of Guelph,Department of Physics and Biophysics Interdepartmental Group

[2] Kobe Pharmaceutical University,Department of Organic Chemistry for Life Science

[3] University of Toronto,Department of Medical Genetics and Microbiology, Medical Sciences Building

来源：

Biomolecular NMR Assignments | 2013年 / 7卷

关键词：

Sensory Rhodopsin; Bacteriorhodopsin; Magic-angle spinning; Solid-state NMR; Chemical shifts; Secondary structure;

D O I：

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学科分类号：

摘要：

Anabaena Sensory Rhodopsin (ASR) is a unique microbial rhodopsin that displays photocromism, interacts with soluble transducer, and may be involved in gene regulation. Here we report nearly complete spectroscopic 13C and 15N assignments of ASR reconstituted in lipids, obtained using two- and three-dimensional magic angle spinning solid state NMR spectroscopy on alternately 13C labeled samples. The obtained chemical shifts are used to characterize the protein backbone conformation. They suggest that lipid-reconstituted ASR has a fold generally similar to that seen in earlier X-ray studies, but with a number of important differences. SSNMR detects double conformations for a number of residues on the cytoplasmic side.

引用

页码：253 / 256

页数：3