Crosslinking of β-cyclodextrin on cholesterol removal from milk

This study was designed to develop crosslinking of β-cyclodextrin (β-CD), and determine the optimum conditions of different factors (mixing time, mixing temperature, and mixing speed) on cholesterol reduction from milk. Crosslinked β-CD was prepared with epichlorohydrin. When milk was treated with different conditions, the cholesterol removal rate was in the range of 79.4 to 83.3% with 1% crosslinked β-CD addition, which were not significantly different among treatments. After cholesterol removal from milk, the used crosslinked β-CD was washed for cholesterol dissociation and reused. For recycling study, the cholesterol removal rate in first trial was 81.8%, which was mostly same as that using new crosslinked β-CD. With five trials repeatedly using the same sample, the mean cholesterol removal rate was 81.2%. The present study indicated that the optimum conditions on cholesterol removal using crosslinked β-CD were 10 min mixing with 400 rpm speed at 5°C with about 80% cholesterol removal. In addition, crosslinked β-CD resulted in the effective recycling efficiency almost 100%.