Yellowing disease of tomatoes caused by Tomato infectious chlorosis virus newly recognized in Japan

被引:12
作者
Sedyo Hartono
Tomohide Natsuaki
Haruki Sayama
Hiroki Atarashi
Seiichi Okuda
机构
[1] Faculty of Agriculture,
[2] Utsunomiya University,undefined
[3] Mine-machi 350,undefined
[4] Utsunomiya 321-8505,undefined
[5] Japan,undefined
[6] Nippon Del Monte Co.,undefined
[7] Numata,undefined
[8] Japan,undefined
关键词
Key words Tomato infectious chlorosis virus; Tomato; Crinivirus; Closterovirus; Yellowing disease;
D O I
10.1007/s10327-002-0015-x
中图分类号
学科分类号
摘要
 In 2001 virus-like symptoms of yellowing, interveinal chlorosis, leaf-rolling, and necrotic fleck were observed on greenhouse- and field-grown tomato plants (Lycopersicon esculentum Miller) in Gunma and Tochigi Prefectures, Japan. Characteristics of the causal agent, such as particle morphology, whitefly transmission, and symptomatology, are similar to those of Tomato infectious chlorosis virus (TICV) and Tomato chlorosis virus, species of the genus Crinivirus in the family Closteroviridae. Electrophoretic analysis of extracted double-stranded RNAs revealed two major species of approximately 7 kbp. Sequencing of reverse transcription-polymerase chain reaction products using the specific primers for heat shock protein 70 homolog and diverged coat protein genes of closteroviruses indicated that the virus was TICV.
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页码:61 / 64
页数:3
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