Eosinophil Cationic Protein Stimulates TGF-β1 Release by Human Lung Fibroblasts In Vitro

被引：0
作者：
Ulrika Zagai
Elham Dadfar
Joachim Lundahl
Per Venge
C. Magnus Sköld
机构：
[1] Division of Respiratory Medicine,Division of Clinical Immunology and Allergy, Department of Medicine, Karolinska Institutet
[2] Department of Medicine,Department of Medical Sciences, Clinical Chemistry
[3] Karolinska Institutet,undefined
[4] Karolinska University Hospital,undefined
[5] University of Uppsala,undefined
来源：
Inflammation | 2007年 / 30卷
关键词：
airway remodeling; ECP; eosinophil; fibroblast; TGF-β;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Eosinophilic inflammation and airway remodeling are features of asthma. Eosinophil cationic protein (ECP) is released by activated eosinophils and transforming growth factor (TGF)-β1 has major functions in the fibrotic process. We therefore hypothesized that ECP stimulates TGF-β1 release by human lung fibroblasts. Fibroblasts in monolayer displayed a constitutive release of TGF-β1, which increased in presence of ECP (436 ± 60 vs. 365 ± 48 pg/ml at 48 h; P < 0.01). mRNA expression of TGF-β1 was almost twofold in ECP-stimulated fibroblasts. ECP in three-dimensional cultures stimulated both TGF-β1 release (180 ± 61 vs. 137 ± 54 pg/ml; P < 0.01) and fibroblast-mediated collagen gel contraction (28 vs. 39% of initial gel area at 48 h; P < 0.001). ECP stimulates TGF-β1-release by human lung fibroblasts, suggesting a potential mechanism for eosinophils in the fibrotic response. This may be an important mechanism by which ECP promotes remodeling of extra cellular matrix leading to airway fibrosis in asthmatics.
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页码：153 / 160
页数：7
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共 120 条
  • [1] Wilson J. W.(2001)Assessing the evidence for remodelling of the airway in asthma Pulm. Pharmacol. Ther. 14 229-247
  • [2] Bamford T. L.(1999)Eosinophil cationic protein (ECP): molecular and biological properties and the use of ECP as a marker of eosinophil activation in disease Clin Exp Allergy 29 1172-1186
  • [3] Venge P.(1996)Eosinophils in allergy: role in disease, degranulation, and cytokines Int Arch Allergy Immunol. 109 207-215
  • [4] Bystrom J.(2004)Monitoring the allergic inflammation Allergy 59 26-32
  • [5] Carlson M.(2004)Wound healing: an overview of acute, fibrotic and delayed healing Front. Biosci. 9 283-289
  • [6] Hakansson L.(2004)The effect of eosinophils on collagen gel contraction and implications for tissue remodelling Clin. Exp. Immunol. 135 427-433
  • [7] Karawacjzyk M.(1979)Production of a tissue-like structure by contraction of collagen lattices by human fibroblasts of different proliferative potential in vitro Proc. Natl. Acad. Sci. U.S.A. 76 1274-1278
  • [8] Peterson C.(1994)Fibroblasts, myofibroblasts, and wound contraction J. Cell Biol. 124 401-404
  • [9] Seveus L.(1998)Fibronectin production by cultured human lung fibroblasts in three-dimensional collagen gel culture In. Vitro. Cell Dev. Biol. Anim. 34 203-210
  • [10] Trulson A.(2000)Activin receptors are expressed on human lung fibroblast and activin A facilitates fibroblast-mediated collagen gel contraction Life Sci. 66 1603-1613
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