Identification and characterization of members of the FKHR (FOX O) subclass of winged-helix transcription factors in the mouse

被引：0

作者：

William H. Biggs III

Webster K. CaveneeKaren C.

机构：

[1] Ludwig Institute for Cancer Research,

[2] San Diego Branch,undefined

[3] La Jolla,undefined

[4] California 92093-0660,undefined

[5] USA,undefined

[6] Department of Medicine,undefined

[7] School of Medicine,undefined

[8] University of California,undefined

[9] San Diego,undefined

[10] La Jolla,undefined

[11] California 92093-0660,undefined

[12] USA,undefined

[13] Center for Molecular Genetics,undefined

[14] University of California,undefined

[15] San Diego,undefined

[16] La Jolla,undefined

[17] California 92093-0660,undefined

[18] USA,undefined

[19] Cancer Center,undefined

[20] University of California,undefined

[21] San Diego,undefined

[22] La Jolla,undefined

[23] California 92093-0660,undefined

[24] USA,undefined

来源：

Mammalian Genome | 2001年 / 12卷

关键词：

Transcription Factor; Human Cancer; Genetic Alteration; Distinct Pattern; Cellular Differentiation;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

Genetic alterations of FKHR (FOXO1), AF6q21 (FOXO2), and AFX (FOXO4), closely related members of the forkhead family of DNA binding proteins, in human cancers has suggested they play a role in the regulation of cellular differentiation or proliferation. In order to elucidate the function of this gene subfamily during mammalian development, we have identified and characterized three novel mouse genes; Fkhr1 (Foxo1), Fkhr2 (Foxo3), and Afxh (Foxo4), which are closely related to the human FKHR (Foxo1), AF6q21 (FOXO2), and AFX (FOXO4) genes, respectively. The genes are each expressed both during development and in the adult with distinct patterns ranging from ubiquitous [Fkhr2 (Foxo3)] to tissue-specific [Afxh (Foxo4)]. Selection of high-affinity DNA-binding sites from a pool of degenerate oligonucleotides demonstrated that the proteins encoded by these genes recognize a core sequence [(T/A) (A/T) A A C A] similar to that recognized by other forkhead domain-containing proteins. We have also identified additional FKHR-related genes expressed during development in both the chick and zebrafish. Further characterization will provide insight into the roles of members of the FKHR subfamily of forkhead-related genes during both normal and neoplastic development.

引用

页码：416 / 425

页数：9