Imaging theory and resolution improvement of two-photon confocal microscopy

被引：0
|
作者：
Tang Zhilie
Yang Chuping
Pei Hongjin
Liang Ruisheng
Liu Songhao
机构：
[1] South China Normal University,Department of Physics
[2] South China Agricultural University,College of Sciences
来源：
Science in China Series A: Mathematics | 2002年 / 45卷
关键词：
two-photon; confocal microscopy; resolution;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
The nonlinear effect of two-photon excitation on the imaging property of two-photon confocal microscopy has been analyzed by the two-photon fluorescence intensity transfer function derived in this paper. The two-photon fluorescence intensity transfer function in a confocal microscopy is given. Furthermore the three-dimensional point spread function (3D-PSF) and the three-dimensional optical transfer function (3D-OTF) of two-photon confocal microscopy are derived based on the nonlinear effect of two-photon excitation. The imaging property of two-photon confocal microscopy is discussed in detail based on 3D-OTF. Finally the spatial resolution limit of two-photon confocal microscopy is discussed according to the uncertainty principle.
引用
收藏
页码：1468 / 1478
页数：10
相关论文
共 50 条
  • [11] Photoluminescent Tomography of Semiconductors by Two-Photon Confocal Microscopy Technique
    V. P. Kalinushkin
    O. V. Uvarov
    A. A. Gladilin
    Journal of Electronic Materials, 2018, 47 : 5087 - 5091
  • [12] Soft computing approach to confocal and two-photon excitation microscopy
    Vicidomini, Giuseppe
    Mondal, Partha P.
    Diaspro, Alberto
    THREE-DIMENSIONAL AND MULTIDIMENSIONAL MICROSCOPY: IMAGE ACQUISITION AND PROCESSING XIV, 2007, 6443
  • [13] Two-colour two-photon confocal microscopy with isotropic three-dimensional resolution and parallel excitation
    Ni, J.
    Qiao, L.
    Wang, C.
    Zhao, F.
    Cheng, Y.
    Xu, Z.
    JOURNAL OF MICROSCOPY, 2009, 234 (02) : 205 - 210
  • [14] Two-photon time-resolved confocal microscopy using a digital micromirror device
    Schellenberg, M.
    Peev, E.
    Kloster, M.
    Napier, J.
    Neu, W.
    EMERGING DIGITAL MICROMIRROR DEVICE BASED SYSTEMS AND APPLICATIONS III, 2011, 7932
  • [15] Microglial morphology determined with confocal and two-photon laser scanning microscopy
    Mitran, Smaranda Ioana
    Burada, Emilia
    Tanasie, Cornelia-Andreea
    Manea, Nicolae Catalin
    Ciorbagiu, Mihai Calin
    Mirea, Cecil Sorin
    Balseanu, Tudor-Adrian
    ROMANIAN JOURNAL OF MORPHOLOGY AND EMBRYOLOGY, 2018, 59 (02) : 485 - 490
  • [16] Two-photon speckle illumination for super-resolution microscopy
    Negash, Awoke
    Labouesse, Simon
    Chaumet, Patrick C.
    Belkebir, Kaman
    Giovannini, Hugues
    Allain, Marc
    Idier, Jerome
    Sentenac, Anne
    JOURNAL OF THE OPTICAL SOCIETY OF AMERICA A-OPTICS IMAGE SCIENCE AND VISION, 2018, 35 (06) : 1028 - 1033
  • [17] A Comparison of Confocal and Two-Photon Microendoscopy
    Meier, R.
    Kromer, K.
    Stepp, H.
    Sroka, R.
    WORLD CONGRESS ON MEDICAL PHYSICS AND BIOMEDICAL ENGINEERING, VOL 25, PT 6, 2009, 25 : 177 - 178
  • [18] Confocal and two-photon imaging in cartilage: expression patterns of Filamin A and B
    Wachsmann-Hogiu, S
    Krakow, D
    Sebald, ET
    Bertolotto, C
    Acuna, D
    Farkas, DL
    IMAGING, MANIPULATION, AND ANALYSIS OF BIOMOLECULES, CELLS, AND TISSUES II, 2004, 5322 : 140 - 145
  • [19] Effects of aberrations and specimen structure in conventional, confocal and two-photon fluorescence microscopy
    Simmonds, R. D.
    Wilson, T.
    Booth, M. J.
    JOURNAL OF MICROSCOPY, 2012, 245 (01) : 63 - 71
  • [20] Testate Amoebae Examined by Confocal and Two-Photon Microscopy: Implications for Taxonomy and Ecophysiology
    Burdikova, Zuzana
    Capek, Martin
    Ostasov, Pavel
    Machac, Jiri
    Pelc, Radek
    Mitchell, Edward A. D.
    Kubinova, Lucie
    MICROSCOPY AND MICROANALYSIS, 2010, 16 (06) : 735 - 746
12345