Anti-TMV Effects of Amaryllidaceae Alkaloids Isolated from the Bulbs of Lycoris radiata and Lycoricidine Derivatives

被引：11
作者：
Yang D.-Q. [1 ,2 ]
Chen Z.-R. [3 ]
Chen D.-Z. [1 ]
Hao X.-J. [1 ]
Li S.-L. [1 ]
机构：
[1] State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming
[2] University of Chinese Academy of Sciences, Beijing
[3] Tianjin Agricultural University, Tianjin
来源：
Natural Products and Bioprospecting | 2018年 / 8卷 / 3期
关键词：
Amaryllidaceae alkaloids; Anti-TMV activity; Lycoricidine derivatives; Lycoris radiata; Tobacco mosaic virus;
D O I：
10.1007/s13659-018-0163-0
中图分类号：
学科分类号：
摘要：
Abstract: Fifteen known amaryllidaceae alkaloids were isolated from the bulbs of Lycoris radiata. Some of the compounds and lycoricidine derivatives had been screened for the activities against tobacco mosaic virus (TMV) by the conventional half-leaf method. Lycoricidine derivatives were also carried out the assay of effect on systemic infection of TMV by western-blot and RT-PCR analysis. The tested compounds showed moderate inactivation effect, whereas the lycoricidine derivatives showed good protective effect. The protective inhibitory activity of compounds L1 (N-methyl-2,3,4-trimethoxylycoricidine) (60.8%) and L3 (N-methyl-2-methoxy-3,4-acetonidelycoricidine) (62.0%) was almost similar to the positive control, Ningnanmycin (66.4%). RT-PCR and Western-blot analysis displayed that compounds L1, L3, L5 (N-allyl-2,3,4-triallyloxylycoricidine) exhibited antiviral activity, which was evidenced by reducing TMV-CP gene replication and TMV-CP protein expression. Additionally, defensive enzyme activities confirmed that compound L1 could increase the activity of PAL, POD, SOD to improve disease resistance of tobacco. Graphical Abstract: [Figure not available: see fulltext.]. © 2018, The Author(s).
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页码：189 / 197
页数：8
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