Multicentre Evaluation of a Commercial Test for the Rapid Diagnosis of Clostridium difficile-Mediated Antibiotic-Associated Diarrhoea

被引：0

作者：

A. H. Bentley

N. B. Patel

M. Sidorczuk

P. Loy

J. Fulcher

P. Dexter

J. Richards

S. P. Borriello

K. W. Zak

E. M. Thorn

机构：

[1] Department of Bacteriology,

[2] Edgware General Hospital,undefined

[3] Burnt Oak Broadway,undefined

[4] Edgware,undefined

[5] Middlesex HA8 0AD,undefined

[6] UK,undefined

[7] Department of Bacteriology,undefined

[8] North Manchester General Hospital,undefined

[9] Delaunys Road,undefined

[10] Manchester M86 RL,undefined

[11] UK,undefined

[12] School of Biological Sciences,undefined

[13] University of East Anglia,undefined

[14] Norwich NR4 7TJ,undefined

[15] UK,undefined

[16] Public Health Laboratory,undefined

[17] Bowthorpe Road,undefined

[18] Norwich,undefined

[19] NR2 3TX,undefined

[20] UK,undefined

[21] Central Public Health Laboratory,undefined

[22] Colindale,undefined

[23] London NW9 5HT,undefined

[24] UK,undefined

[25] Unipath Ltd.,undefined

[26] Priory Business Park,undefined

[27] Bedford MK44 3UP,undefined

[28] UK e-mail: kris.zak@unilever.com,undefined

来源：

European Journal of Clinical Microbiology and Infectious Diseases | 1998年 / 17卷

关键词：

Cytotoxicity Assay; Stool Sample; Minimal Sample; Clostridium Difficile; Cell Cytotoxicity;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

An immunoassay for the detection of Clostridium difficile toxin A in stool samples (Clearview C. DIFF A; Unipath, UK) was evaluated against the cell cytotoxicity assay using 407 stool samples from patients suspected to have, or considered at risk of, antibiotic-associated diarrhoea. Of the samples tested, 98 were positive and 280 were negative by both tests (sensitivity 83.1%, specificity 96.9%). Following resolution of the 29 discrepant results, the sensitivity and specificity of the immunoassay were 91% and 98%, respectively, and the sensitivity for the cell cytotoxicity assay was calculated as 91.5%, with a specificity of 99%. The Clearview C. DIFF A test proved to be a rapid simple assay for the detection of Clostridium difficile toxin A in stool samples. The test was equally suited to single or batch testing, required minimal sample handling, and provided results within 30 min of applying the sample to the test unit.

引用

页码：788 / 790

页数：2