A Novel ABA-Responsive TaSRHP Gene from Wheat Contributes to Enhanced Resistance to Salt Stress in Arabidopsis thaliana

A microarray analysis of the salt-resistant wheat mutant, RH8706-49, revealed a salt-induced gene containing a conserved DUF581 domain. The gene was cloned and designated as Triticum aestivum salt-related hypothetical protein (TaSRHP) and submitted to GenBank (accession no. GQ476575). Over-expression of TaSRHP in wild-type Arabidopsis thaliana cv. Columbia resulted in enhanced resistance to both salt and drought stresses. The sensitivity of the transgenic A. thaliana to abscisic acid (ABA) was also increased compared to that of wild-type plants. Furthermore, transgenic plants accumulated more K+ and proline and had a higher osmotic potential and lower Na+ content than untransformed plants. Real-time quantitative PCR analysis indicated that expression of TaSRHP was affected by salt, drought, cold, ABA, and other stresses, and expression of other stress-related genes in the transgenic plants differed from those of the control. Results indicate that the wheat TaSRHP gene may enhance the tolerance of plants to multiple abiotic stresses.