A new Rhizoctonia sp. closely related to Waitea circinata causes a new disease of creeping bentgrass

被引：16

作者：

Toda T. ^{[1
,4
]}

Hayakawa T. ^{[2
]}

Mghalu J.M. ^{[3
]}

Yaguchi S. ^{[2
]}

Hyakumachi M. ^{[1
]}

机构：

[1] Laboratory of Plant Pathology, Faculty of Applied Biological Science, Gifu University, Gifu 501-1193

[2] Research Institute of Riken Green Co., Ltd, Iwata, Shizuoka 437-1218

[3] United Graduate School of Agricultural Science, Gifu University, Gifu 501-1193

[4] Department of Plant Pathology, North Carolina State University, Center for Fungal Research, Raleigh

来源：

Journal of General Plant Pathology | 2007年 / 73卷 / 6期

关键词：

Agrostis stolonifera var. palustris; Poa pratensis; Rhizoctonia oryzae; Rhizoctonia zeae; Waitea circinata;

D O I：

10.1007/s10327-007-0045-5

中图分类号：

学科分类号：

摘要：

Isolates of an unidentified Rhizoctonia sp. (UR isolates) were obtained from creeping bentgrass and Kentucky bluegrass with reddish brown sheath and foliar rots. Because the UR isolates anastomosed with isolates of three varieties of Waitea circinata (var. oryzae, var. zeae, and var. circinata), colony morphology, hyphal growth rate at different temperatures, pathogenicity, sequence analysis of the internal transcribed spacers (ITS) region of ribosomal RNA genes (rDNA) were compared. The colony color of mature UR isolates was distinct from isolates of the other three varieties of W. circinata. In pathogenicity tests on creeping bentgrass, the severity of the disease caused by UR isolates was significantly higher than that caused by the three varieties of W. circinata. Sequence similarities of the rDNA-ITS region between UR isolates and between isolates within each variety were high (97-100%), but they were lower among isolates from UR and the varieties of W. circinata (88-94%). In a phylogenetic tree based on the rDNA-ITS sequences, UR isolates formed a cluster separate from each of the clusters formed by the three varieties of W. circinata. These results indicate that the UR isolates clearly differ from the three varieties of W. circinata. We therefore propose that the UR isolates be classified as new Rhizoctonia sp. that are closely related to W. circinata and that the disease on creeping bentgrass should be called Waitea reddish-brown patch disease (Sekikasshoku-hagusare-byo in Japanese). © 2007 The Phytopathological Society of Japan and Springer.

引用

页码：379 / 387

页数：8

共 24 条

[1]

Bandoni R.J., Safranin O as a rapid nuclear stain for fungi, Mycologia, 71, pp. 873-874, (1979)

[2]

Boysen M., Borja M., Delmont C., Salazar O., Rubio V., Identification at strain level Rhizoctonia solani AG 4 isolates by different sequence of asymmetric PCR products of the regions, Curr Genet, 29, pp. 174-181, (1996)

[3]

Burpee L., Martin B., Biology of Rhizoctonia species associated with turfgrasses, Plant Dis, 76, pp. 112-117, (1992)

[4]

Carling D.E., Sneh K., Jabaji-Hare S., Neate S., Dijst G., Grouping in Rhizoctonia solani by hyphal anastomosis, Rhizoctonia Species: Taxonomy, Molecular Biology, Ecology, Pathology, and Disease Control, pp. 37-47, (1996)

[5]

Erper I., Karaca G.H., Turkkan M., Ozkoc I., Characterization and pathogenicity of Rhizoctonia spp. from onion in amasya, J Phytopathol, 154, pp. 75-79, (2006)

[6]

Gonzalez D., Carling D.E., Kuninaga S., Vilgalys R., Cubeta M.A., Ribosomal DNA systematics of Ceratobasidium and Thanatephorus with Rhizoctonia anamorphs, Mycologia, 93, pp. 1138-1150, (2001)

[7]

Gunnell P.S., Characterization of the Teleomorphs of Rhizoctonia oryzae-sativae, Rhizoctonia oryzae, and Rhizoctonia zeae, and the Effect of Cultural Practices on Aggregate Sheath Spot of Rice, Caused by R. oryzae-sativae, (1986)

[8]

Hsiang T., Dean J.D., DNA sequencing for anastomosis grouping of Rhizoctonia solani isolates from Poa annua, Int Turfgrasss Soc Res J, 9, pp. 674-678, (2001)

[9]

Johanson A., Turner H.C., McKay G.J., Brown A.E., A PCR-based method to distinguish fungi of the rice sheath-blight complex, Rhizoctonia solani, R. oryzae and R. oryzae-sativae, FEMS Microbiol Lett, 162, pp. 289-294, (1998)

[10]

Kuninaga S., Natsuaki T., Takeuchi T., Yokosawa K., Sequence variation of the rDNA ITS regions within and between anastomosis groups in Rhizoctonia solani, Curr Genet, 32, pp. 237-243, (1997)

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