C-banded karyotype of Thinopyrum bessarabicum and identification of its chromosomes in wheat background

被引：0
作者：
G. Mirzaghaderi
H. Shahsevand Hassani
G. Karimzadeh
机构：
[1] University of Kurdistan,Agronomy and Plant Breeding Department, Faculty of Agriculture
[2] University of Shahid Bahonar,Plant Breeding Department, Faculty of Agriculture
[3] Tarbiat Modares University,Plant Breeding Department, Faculty of Agriculture
来源：
Genetic Resources and Crop Evolution | 2010年 / 57卷
关键词：
C-banding; Wheat;
D O I：
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中图分类号：
学科分类号：
摘要：
C-banded pattern in two accessions of Thinopyrum bessarabicum (Save ex Rayss) A. Löve (2n = 2x = 14, EbEb) and their idiogram was established. C-banding analysis was further used to identify the chromosomes of Tritipyrum amphiploid (2n = 6x = 42, AABBEbEb) and a BC1F2 genotype from wheat and Tritipyrum. Two 18S-26S rDNA loci were detected on Th. bessarabicum chromosomes by in situ hybridization using an 18S-26S rDNA probe. Eb chromosomes in Tritipyrum generally were identified by their distinctive C-banding patterns which reflected heterochromatin regions. C-banding procedure resulted in sharp and distinct bands in one or both ends of Eb chromosomes without interval bands. Observed C-bands in Eb genome mainly reflected the telomeric and subtelomeric sequences which also showed more strong signals in genomic in situ hybridization. Results showed the importance of the C-banding technique as a screening tool in identification of addition and substitution lines in the progenies of wheat and Tritipyrum crosses during segregating generations.
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页码：319 / 324
页数：5
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