Development of an experimental model for radiation-induced inhibition of cranial bone regeneration

被引：4
作者：
Jung H.-M. [1 ,2 ]
Lee J.-E. [3 ]
Lee S.-J. [4 ]
Lee J.-T. [1 ]
Kwon T.-Y. [5 ]
Kwon T.-G. [1 ]
机构：
[1] Department of Oral and Maxillofacial Surgery, School of Dentistry, Kyungpook National University, 2177 Dalgubeol-daero, Jung-gu, Daegu
[2] Department of Radiologic Technology, Daegu Health College, Taejeon-Dong 15, Youngsong-Ro, Buk-Gu, Daegu
[3] Department of Radiation Oncology, School of Medicine, Kyungpook National University, 130 Dongdeok-ro, Jung-gu, Daegu
[4] Department of Radiation Oncology, Kyungpook National University Hospital, 130 Dongdeok-ro, Jung-gu, Daegu
[5] Department of Dental Materials, School of Dentistry, Kyungpook National University, 2177 Dalgubeol-daero, Jung-gu, Daegu
来源：
Maxillofacial Plastic and Reconstructive Surgery | / 40卷 / 1期
基金：
新加坡国家研究基金会;
关键词：
Bone; Calvaria; Defect; Radiation; Regeneration;
D O I：
10.1186/s40902-018-0173-1
中图分类号：
学科分类号：
摘要：
Background: Radiation therapy is widely employed in the treatment of head and neck cancer. Adverse effects of therapeutic irradiation include delayed bone healing after dental extraction or impaired bone regeneration at the irradiated bony defect. Development of a reliable experimental model may be beneficial to study tissue regeneration in the irradiated field. The current study aimed to develop a relevant animal model of post-radiation cranial bone defect. Methods: A lead shielding block was designed for selective external irradiation of the mouse calvaria. Critical-size calvarial defect was created 2 weeks after the irradiation. The defect was filled with a collagen scaffold, with or without incorporation of bone morphogenetic protein 2 (BMP-2) (1 μg/ml). The non-irradiated mice treated with or without BMP-2-included scaffold served as control. Four weeks after the surgery, the specimens were harvested and the degree of bone formation was evaluated by histological and radiographical examinations. Results: BMP-2-treated scaffold yielded significant bone regeneration in the mice calvarial defects. However, a single fraction of external irradiation was observed to eliminate the bone regeneration capacity of the BMP-2-incorporated scaffold without influencing the survival of the animals. Conclusion: The current study established an efficient model for post-radiation cranial bone regeneration and can be applied for evaluating the robust bone formation system using various chemokines or agents in unfavorable, demanding radiation-related bone defect models. © 2018, The Author(s).
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