MMP 9 gene promoter polymorphism in gastric cancer

被引：13

作者：

Krishnaveni D. ^{[1
]}

Bhayal A.C. ^{[1
]}

Manjari K.S. ^{[1
]}

Vidyasagar A. ^{[2
]}

Devi M.U. ^{[2
]}

Ramanna M. ^{[2
]}

Jyothy A. ^{[1
]}

Nallari P. ^{[3
]}

Venkateshwari A. ^{[1
]}

机构：

[1] Institute of Genetics, Hospital for Genetic Diseases, Osmania University, Begumpet

[2] Department of Gastroenterology, Gandhi Hospital

[3] Department of Genetics, Osmania University

来源：

Indian Journal of Clinical Biochemistry | 2012年 / 27卷 / 3期

关键词：

Gastric cancer; Matrix metalloproteinase-9; Metastasis; Polymorphism;

D O I：

10.1007/s12291-012-0210-2

中图分类号：

学科分类号：

摘要：

Matrix metalloproteinase-9 (gelatinase B) plays a key role in cancer invasion and metastasis by degrading the extracellular matrix and basement membrane barriers. A cytosine (C)[thymidine (T) single nucleotide polymorphism (SNP) at position -1562 in the MMP-9 promoter is reported to influence the expression of the gene. Genotyping of MMP-9 -1562 C→T promoter polymorphism in 140 gastric cancer patients and 132 healthy control subjects was carried out in order to evaluate its association with progression and development of gastric cancer. The SNP was genotyped by tetra-primer amplification refractory mutation system-polymerase chain reaction followed by agarose gel electrophoresis. Statistical methods were adopted to test for the significance of the results. Risk factor profile of the patients revealed age above 50 years, smoking, alcoholism as the factors associated with the disease. The distribution of genotype frequencies in gastric cancer patients were 28.7 % of CC, 45.5 % of CT and 25.7 % of TT, whereas in control subjects 31.8 % of CC, 53.03 % of CT and 15.15 % of TT, respectively. The allelic frequencies were 51.51 % of C and 48.48 %of T in patient group and 58.33 %of C and 41.66 % of T in controls respectively. The present study shows the possible association of epidemiological risk factors with gastric cancer. There is an increased frequency of T allele in the disease compared to control subjects. However, there is no association of the MMP-9 -1562 C→T promoter polymorphism in the development of gastric cancer. © Association of Clinical Biochemists of India 2012.

引用

页码：259 / 264

页数：5

共 30 条

[1]

Lauren C.C.R., Evely C.F.S., Da Diana Mendes F., Mariana F.L., Adriana C.G., Danielle Q.C., Interrelationship between myc gene numerical aberrations and protein expression in individuals from northern brazil with early gastric adenocarcinoma, Cancer Genet Cytogenet, 181, 1, pp. 31-35, (2008)

[2]

Schwartz G., Invasion and metastasis in gastric cancer: In vitro and in vivo models with clinical considerations, Semin Oncol, 23, pp. 316-324, (1996)

[3]

Neugut A.I., Hayek M., Howe G., Epidemiology of gastric cancer, Semin Oncol, 23, pp. 281-291, (1996)

[4]

Sun H.K., Tae E.J., Ji U.K., Kye C.K., Jong W.P., Seung M.N., Prognostic implications for gastric carcinoma based on loss of heterozygosity genotypes correlation with clinicopathologic variables, Cancer Genet Cytogenet, 153, pp. 26-31, (2004)

[5]

Folgueras A.R., Pendas A.M., Sanchez L.M., Lopez-Otin C., Matrix metalloproteinases in cancer: From new functions to improved inhibition strategies, Int J Dev Biol, 48, pp. 411-424, (2004)

[6]

Hibbs M.S., Hoidal J.R., Kang A.H.J., Expression of a metalloproteinase that degrades native type v collagen and denatured collagens by cultured human alveolar macrophages, Clin Invest, 80, pp. 1644-1650, (1987)

[7]

Wilhelm S.M., Collier I.E., Marmer B.L., Eisen A.Z., Grant G.A., Goldberg G.I., Sv40-Transformed human lung fibroblasts secrete a 92 kda type iv collagenase which is identical to that secreted by normal human macrophages, J Biol Chem, 264, pp. 17213-17221, (1989)

[8]

Murphy G., Ward R., Hembry R.M., Reynolds J.J., Kuhn K., Tryggvason K., Characterization of gelatinase from pig polymorphonuclear leucocytes. A metalloproteinase resembling tumour type iv collagenase, Biochem J, 258, pp. 463-472, (1989)

[9]

Senior R.M., Griffin G.L., Fliszar C.J., Shapiro S.D., Goldberg G.I., Welgus H.G., Human 92- and 72-kilodalton type iv collagenases are elastases, J Biol Chem, 266, pp. 7870-7875, (1991)

[10]

Welgus H.G., Campbell E.J., Cury J.D., Eisen A.Z., Senior R.M., Wilhelm S.M., Et al., Neutral metalloproteinases produced by human mononuclear phagocytes. Enzyme profile, regulation, and expression during cellular development, J Clin Invest, 86, pp. 1496-1502, (1990)

← 1 2 3 →