RNA live imaging in microorganisms

被引:0
|
作者
Müntjes K. [1 ]
Feldbrügge M. [1 ]
机构
[1] Institut für Mikrobiologie, Cluster of Excellence on Plant Sciences, Heinrich-Heine-Universität Düsseldorf, Universitätsstraße 1, Düsseldorf
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D O I
10.1007/s12268-020-1323-4
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学科分类号
摘要
RNA live imaging is a powerful technique to visualize the subcellular localization as well as highly dynamic transport of mRNAs in vivo. Established in eukaryotic microorganisms, it is now a wide-spread system used in bacteria, animals, and plants. The method is based on the binding of heterologous RNA-binding proteins to their cognate binding sites that are integrated into the mRNA of interest. Recent advances enable the visualization of transcriptional or translational sites as well as mRNA decay. © 2020, Die Autoren.
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页码:37 / 39
页数:2
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