Differential Gene Expression of Human CD34+ Hematopoietic Stem and Progenitor Cells Before and After Culture

被引：0
|
作者：
Qunliang Li
Haibo Cai
Qiwei Liu
Wen-Song Tan
机构：
[1] East China University of Science and Technology,The State Key Laboratory of Bioreactor Engineering
来源：
Biotechnology Letters | 2006年 / 28卷
关键词：
cDNA array; CD34; hematopoietic stem cells; cord blood; expansion; SMART-PCR;
D O I：
暂无
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学科分类号：
摘要：
Ex vivo expanded CD34+ hematopoietic stem and progenitor cells (HSPCs) have compromised homing and engraftment capacities. To investigate underlying mechanisms for functional changes of expanded HSPCs, we compared gene expression profiling of cultured and fresh CD34+ cells derived from cord blood using SMART-PCR and cDNA array: 20 genes were up-regulated while 25 genes were down-regulated in cultured CD34+ HSPCs. These differentially expressed genes are involved primarily in proliferation, differentiation, apoptosis, and homing.
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页码：389 / 394
页数：5
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