Three consecutive crops of malting barley grown during 2002–2004 on clay-loam on a Swedish farm (59°74’ N, 17°00’ E) were monitored for canopy reflectance at growth stages GS32 (second node detectable) and GS69 (anthesis complete), and the crops were sampled for above ground dry matter and nitrogen content. GPS-positioned unfertilised plots were established and used for soil sampling. At harvest, plots of 0.25 m2 were cut in both fertilised and unfertilised plots, and 24 m2 areas were also harvested from fertilised barley. The correlations between nine different vegetation indices (VIs) from each growth stage and yield and grain protein were tested. All indices were significantly correlated (at 5% level) with grain yield (GY), and protein when sampled at GS69 but only four when sampled at GS32. Three variables (the best-correlated vegetation index sampled at GS32; an index for accumulated elevated daily maximum temperatures for the grain filling period, and normalised apparent electrical conductivity (ECa) of the soil) were sufficient input in the final regressions. Using these three variables, it was possible to make either one multivariate (PLS) regression model or two linear multiple regression models for grain yield (GY) and grain protein, with correlation coefficients of 0.90 and 0.73 for yield and protein, respectively.
