Low power laser stimulation of the bone consolidation in tibial fractures of rats: a radiologic and histopathological analysis

被引：0
作者：
M. Briteño-Vázquez
G. Santillán-Díaz
M. González-Pérez
T. Gallego-Izquierdo
D. Pecos-Martín
G. Plaza-Manzano
N. Romero-Franco
机构：
[1] Benemérita Autonomous University of Puebla,Physiotherapy and Nursery Department
[2] Veterinarian Hospital of Small Species of Benemérita Autonomous University of Puebla,Nursery Department
[3] Biomedical Engineering. Popular Autonomous University of Puebla,Rehabilitation and Physical Medicine Department. Medical hydrology
[4] University of Alcalá,Physiotherapy and Nursery Department
[5] University of Alcalá,undefined
[6] Complutense University,undefined
[7] University of Alcalá,undefined
来源：
Lasers in Medical Science | 2015年 / 30卷
关键词：
Low power laser stimulation; Bone consolidation; Shinbone fractures; Rats;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
The objective of this study is to analyze the effectiveness of low power laser irradiation in the bone consolidation of tibial fractures in rats. An experimental, comparative, prospective study with control group was designed. Twenty Wistar rats were grouped into control (n = 10) and experimental groups (n = 10). A tibial fracture, with a mechanical drill, was inflicted in all rats. The experimental group received ten days of low power arsenide-gallium laser irradiation of 850 nm (KLD, Sao Paulo, Brasil)—100 mW, 8 J/cm2, 64 s. Before and after the laser treatment, a radiologic analysis was carried out in both groups, in which the rats were graded from 0 to IV according the Montoya scale of bone consolidation. Also, we histopathologically analyzed the bone to estimate the proliferation of fibroblasts, bone matrix, and angiogénesis with a microscopy, which were graded as I (thin layer of fibroblasts and osteoid matrix), II (thick layer of fibroblasts and osteoid matrix), or III (thick layer of fibroblasts and osteoid matrix and new blood vessels). Radiologic data showed that the experimental group had a higher bone consolidation of Montoya scale after ten days of laser irradiation compared to control group (P < 0.004). Histopathologic data showed more fibroblasts and angiogenesis presence in the group receiving laser irradiation, compared to control group (P < .002). The low power laser radiation therapy may expedite the bone repair after tibial fractures in rats, according to radiologic and histopathologic analysis.
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页码：333 / 338
页数：5
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