Expression and purification of soluble recombinant β-lactamases using Escherichia coli as expression host and pET-28a as cloning vector

被引：0
作者：
Lele Li
Hui Li
Qingwu Tian
Baosheng Ge
Xiaotong Xu
Yuanyuan Chi
Huaizhi Zhao
Yanfei Liu
Nan Jia
Tingting Zhou
Yuanqi Zhu
Yusun Zhou
机构：
[1] The Affiliated Hospital of Qingdao University,Department of Clinical Laboratory
[2] China University of Petroleum (East China),State Key Laboratory of Heavy Oil Processing and Center for Bioengineering and Biotechnology
[3] The Fourth Affiliated Hospital Zhejiang University School of Medicine,Department of Laboratory Medicine
[4] The Affiliated Hospital of Qingdao University,Department of Pediatric Emergency
[5] Qingdao Women and Children’s Hospital,undefined
来源：
Microbial Cell Factories | / 21卷
关键词：
Recombinant β-lactamases; Soluble protein expression; Protein affinity purification; Recombinant pET-28a plasmids; BL21 (DE3);
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