Affinity purification–mass spectrometry and network analysis to understand protein-protein interactions

被引:0
|
作者
John H Morris
Giselle M Knudsen
Erik Verschueren
Jeffrey R Johnson
Peter Cimermancic
Alexander L Greninger
Alexander R Pico
机构
[1] University of California,Department of Pharmaceutical Chemistry
[2] San Francisco,Department of Cellular and Molecular Pharmacology
[3] University of California,undefined
[4] San Francisco,undefined
[5] Graduate Group in Bioinformatics,undefined
[6] University of California,undefined
[7] San Francisco,undefined
[8] School of Medicine,undefined
[9] University of California,undefined
[10] San Francisco,undefined
[11] Gladstone Institutes,undefined
[12] University of California,undefined
[13] San Francisco,undefined
来源
Nature Protocols | 2014年 / 9卷
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学科分类号
摘要
From proteomics to networks, this protocol covers the processing, analysis and interpretation of AP-MS data. Sample data are pre-processed and scored using a variety of methods, and they are then imported into Cytoscape for network analysis and visualization.
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页码:2539 / 2554
页数:15
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