Affinity purification–mass spectrometry and network analysis to understand protein-protein interactions

被引：0
|
作者：
John H Morris
Giselle M Knudsen
Erik Verschueren
Jeffrey R Johnson
Peter Cimermancic
Alexander L Greninger
Alexander R Pico
机构：
[1] University of California,Department of Pharmaceutical Chemistry
[2] San Francisco,Department of Cellular and Molecular Pharmacology
[3] University of California,undefined
[4] San Francisco,undefined
[5] Graduate Group in Bioinformatics,undefined
[6] University of California,undefined
[7] San Francisco,undefined
[8] School of Medicine,undefined
[9] University of California,undefined
[10] San Francisco,undefined
[11] Gladstone Institutes,undefined
[12] University of California,undefined
[13] San Francisco,undefined
来源：
Nature Protocols | 2014年 / 9卷
关键词：
D O I：
暂无
中图分类号：
学科分类号：
摘要：
From proteomics to networks, this protocol covers the processing, analysis and interpretation of AP-MS data. Sample data are pre-processed and scored using a variety of methods, and they are then imported into Cytoscape for network analysis and visualization.
引用
收藏
页码：2539 / 2554
页数：15
相关论文
共 50 条
  • [21] Cross-linking Mass Spectrometry Analysis of the Yeast Nucleus Reveals Extensive Protein-Protein Interactions Not Detected by Systematic Two-Hybrid or Affinity Purification-Mass Spectrometry
    Bartolec, Tara K.
    Smith, Daniela-Lee
    Pang, Chi Nam Ignatius
    Xu, You Dan
    Hamey, Joshua J.
    Wilkins, Marc R.
    ANALYTICAL CHEMISTRY, 2020, 92 (02) : 1874 - 1882
  • [22] Protocol for mapping differential protein-protein interaction networks using affinity purification-mass spectrometry
    Kaushal, Prashant
    Ummadi, Manisha R.
    Jang, Gwendolyn M.
    Delgado, Yennifer
    Makanani, Sara K.
    Alba, Kareem
    Winters, Declan M.
    Blanc, Sophie F.
    Xu, Jiewei
    Polacco, Benjamin
    Zhou, Yuan
    Stevenson, Erica
    Eckhardt, Manon
    Zuliani-Alvarez, Lorena
    Kaake, Robyn
    Swaney, Danielle L.
    Krogan, Nevan J.
    Bouhaddou, Mehdi
    STAR PROTOCOLS, 2024, 5 (04):
  • [23] ANALYSIS OF COMPUTATIONALLY DESIGNED COOPERATIVE PROTEIN-PROTEIN INTERACTIONS BY NATIVE MASS SPECTROMETRY
    Busch, Florian
    VanAerum, Zachary
    Jia, Mengxuan
    Chen, Zibo
    Baker, David
    Wysocki, Vicki
    PROTEIN SCIENCE, 2019, 28 : 86 - 87
  • [24] Protein arrays as tools for detection of protein-protein interactions by mass spectrometry
    Becker, Christian F. W.
    Wacker, Ron
    Bouschen, Werner
    Seidel, Ralf P.
    Kolaric, Branko
    Lang, Pascal
    Schroeder, Hendrik
    Niemeyer, Christof
    Spengler, Bernhard
    Goody, Roger S.
    Engelhard, Martin
    UNDERSTANDING BIOLOGY USING PEPTIDES, 2006, : 725 - +
  • [25] Predicting direct protein interactions from affinity purification mass spectrometry data
    Ethan DH Kim
    Ashish Sabharwal
    Adrian R Vetta
    Mathieu Blanchette
    Algorithms for Molecular Biology, 5
  • [26] Predicting direct protein interactions from affinity purification mass spectrometry data
    Kim, Ethan D. H.
    Sabharwal, Ashish
    Vetta, Adrian R.
    Blanchette, Mathieu
    ALGORITHMS FOR MOLECULAR BIOLOGY, 2010, 5
  • [27] Reliable identification of protein-protein interactions by crosslinking mass spectrometry
    Lenz, Swantje
    Sinn, Ludwig R.
    O'Reilly, Francis J.
    Fischer, Lutz
    Wegner, Fritz
    Rappsilber, Juri
    NATURE COMMUNICATIONS, 2021, 12 (01)
  • [28] Identification of Protein-Protein Interactions by Mass Spectrometry Coupled Techniques
    Abu-Farha, Mohamed
    Elisma, Fred
    Figeys, Daniel
    PROTEIN - PROTEIN INTERACTION, 2008, 110 : 67 - 80
  • [29] Reliable identification of protein-protein interactions by crosslinking mass spectrometry
    Swantje Lenz
    Ludwig R. Sinn
    Francis J. O’Reilly
    Lutz Fischer
    Fritz Wegner
    Juri Rappsilber
    Nature Communications, 12
  • [30] Protein-protein interactions of tandem affinity purification-tagged protein kinases in rice
    Rohila, JS
    Chen, M
    Chen, S
    Chen, J
    Cerny, R
    Dardick, C
    Canlas, P
    Xu, X
    Gribskov, M
    Kanrar, S
    Zhu, JK
    Ronald, P
    Fromm, ME
    PLANT JOURNAL, 2006, 46 (01): : 1 - 13
12345