Stimulatory actions of lysophosphatidic acid on mouse ATDC5 chondroprogenitor cells

被引：0
作者：
Ryota Itoh
Shigenori Miura
Aki Takimoto
Shunya Kondo
Hiroko Sano
Yuji Hiraki
机构：
[1] Kyoto University,Department of Cellular Differentiation, Institute for Frontier Medical Sciences
来源：
Journal of Bone and Mineral Metabolism | 2010年 / 28卷
关键词：
ATDC5 cells; Lysophosphatidic acid; Sphingosine-1-phosphate; Cell migration; Chondroprogenitor cells;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Lysophosphatidic acid (LPA) and sphingosine-1-phosphate (S1P) are bioactive lysophospholipids that affect various cellular processes through G protein-coupled receptors. In our current study, we found by in situ hybridization that E11.5 mouse embryos strongly expressed the LPA receptor subtype LPA1 in cartilaginous bone primordia and the surrounding mesenchymal cells. However, despite their wide-ranging actions, the roles of lysophospholipids in chondrogenesis remain poorly understood. The mouse clonal cell line ATDC5 undergoes a sequential differentiation of chondroprogenitor cells in vitro. Undifferentiated and differentiated ATDC5 cells express LPA1 and other lysophospholipid receptors including S1P receptor S1P1 and S1P2. Taking advantage of this cell model, we studied the effects of LPA on the activities of chondroprogenitor cells. LPA markedly stimulates both DNA synthesis and the migration of ATDC5 chondroprogenitor cells in culture, whereas S1P suppresses the migration of these cells. Treatment with Ki16425, an LPA1- and LPA3-specific receptor antagonist, suppressed the fetal bovine serum-stimulated migration of ATDC5 cells by almost 80%. These results indicate that LPA plays an important role in the activation of chondroprogenitor cells.
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页码：659 / 671
页数：12
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共 263 条
  • [11] Spiegel S(2006)Autotaxin stabilizes blood vessels and is required for embryonic vasculature by producing lysophosphatidic acid J Biol Chem 281 25822-25830
  • [12] Bachner D(2000)Sphingosine 1-phosphate signalling via the endothelial differentiation gene family of G-protein-coupled receptors Pharmacol Ther 88 115-131
  • [13] Ahrens M(2003)Molecular mechanisms of lysophosphatidic acid action Prog Lipid Res 42 498-526
  • [14] Betat N(2000)Genomic characterization of the lysophosphatidic acid receptor gene, lp(A2)/Edg4, and identification of a frameshift mutation in a previously characterized cDNA Genomics 64 155-169
  • [15] Schroder D(2003)Sphingosine-1-phosphate: an enigmatic signalling lipid Nat Rev Mol Cell Biol 4 397-407
  • [16] Gross G(2003)Identification of p2y9/GPR23 as a novel G protein-coupled receptor for lysophosphatidic acid, structurally distant from the Edg family J Biol Chem 278 25600-25606
  • [17] Ohuchi H(2006)Lysophosphatidic acid binds to and activates GPR92, a G protein-coupled receptor highly expressed in gastrointestinal lymphocytes J Pharmacol Exp Ther 318 619-628
  • [18] Hayashibara Y(2006)GPR92 as a new G12/13- and Gq-coupled lysophosphatidic acid receptor that increases cAMP, LPA5 J Biol Chem 281 23589-23597
  • [19] Matsuda H(2007)The orphan GPCR GPR87 was deorphanized and shown to be a lysophosphatidic acid receptor Biochem Biophys Res Commun 363 861-866
  • [20] Onoi M(2008)G protein-coupled receptor P2Y5 and its ligand LPA are involved in maintenance of human hair growth Nat Genet 40 329-334
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