CD95 (APO-1/Fas) expression on naive CD4+ T cells increases with disease progression in HIV-infected children and adolescents: Effect of highly active antiretroviral therapy (HAART)

被引：10
作者：
Böhler T. [1 ]
Wintergerst U. [1 ]
Linde R. [1 ]
Belohradsky B.H. [1 ]
Debatin K.-M. [1 ]
机构：
[1] University Children's Hospital, 89075 Ulm
来源：
Pediatric Research | 2001年 / 49卷 / 1期
关键词：
D O I：
10.1203/00006450-200101000-00021
中图分类号：
学科分类号：
摘要：
We studied the expression of the CD95 receptor (APO-1/Fas) on peripheral blood T cell subpopulations in 37 HIV-1-infected children and adolescents stratified according to disease stage or antiretroviral treatment regimen and compared the results to values obtained in 12 healthy age-matched control subjects. CD95 expression on CD45RA+ CD45RO-/CD62L+ (resting/naive) and CD45RO+ CD45RA- (primed/memory) CD4+ and CD8+ T cells was assessed quantitatively by four-color and three-color flow cytometry. CD4+ T cells contained a population of predominantly CD95- resting/naive cells and a population of CD95high primed/memory cells, whereas CD8+ T cells had a more uniform pattern of CD95 expression. The percentage of CD95+ CD4+ T cells increased with disease progression because of both an augmented median fluorescence intensity on resting/naive cells and an increased percentage of CD95high cells. Patients with highly active antiretroviral combination therapy who maintained stable CD4 counts in the presence of elevated plasma viral load had nearly normal numbers of CD95- resting/naive CD4+ T cells, whereas CD95 expression in the CD8+ T cell subset was still elevated compared with control subjects. Low CD95 expression on resting/naive CD4+ T cells may therefore indicate a low risk for disease progression in antiretrovirally treated and untreated patients.
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页码：101 / 110
页数：9
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