Dealing with different methods for Kluyveromyces lactis β-galactosidase purification

被引：16

作者：

Becerra M. ^{[1
]}

Cerdán E. ^{[1
]}

González Siso M.I. ^{[1
]}

机构：

[1] Departamento de Bioloxía Celular e Molecular, Facultade de Ciencias, 15071-A Coruña, Campus da Zapateira s/n

来源：

Biological Procedures Online | 1998年 / 1卷 / 1期

关键词：

Lactase; Cheese Whey; Purification Factor; Biological Procedure; Crude Protein Extract;

D O I：

10.1251/bpo4

中图分类号：

学科分类号：

摘要：

Several micro-scale chromatography-based procedures for purification of the β-galactosidase from the yeast Kluyveromyces lactis were assayed. Purified enzyme was suitable to be used as antigen to induce polyclonal antibodies production. Specific staining of non-denaturing PAGE gels with chromogenic substrates allowed the determination of the number of subunits forming the native enzyme. ©1998 Biological Procedures Online. All rights reserved.

引用

页码：48 / 58

页数：10

共 19 条

[1]

Gonzalez Siso M.I., The biotechnological utilization of cheese whey: A review, Biores.Technol., 57, pp. 1-11, (1996)

[2]

Gonzalez Siso M.I., β-galactosidase production by Kluyveromyces lactis on milk whey: Batch versus fed-batch cultures, Proc. Biochem., 29, pp. 565-568, (1994)

[3]

Becerra M., Gonzalez Siso M.I., Yeast β-galactosidase in solid-state fermentations, Enz. Microb.Technol., 19, pp. 33-44, (1996)

[4]

Gonzalez Siso M.I., Freire Picos A., Ramil E., Rodriguez Belmonte E., Rodriguez Torres A., Cerdan E., Covalent immobilization of β-galactosidase on corn grits.A system for lactose hydrolysis without diffusional resistance, Proc. Biochem., 29, pp. 7-12, (1994)

[5]

Gonzalez Siso M.I., Cerdan E., Freire Picos M.A., Rodriguez Belmonte E., Rodriguez Torres A.M., Permeabilization of Kluyveromyces lactis cells for milk whey saccharification: A comparison of different treatments, Biotechnol. Techniques, 6, pp. 289-292, (1992)

[6]

Gonzalez Siso M.I., Suarez Doval S., Kluyveromyces lactis immobilization on corn grits for milk whey lactose hydrolysis, Enzyme Microb. Technol., 16, pp. 303-310, (1994)

[7]

Becerra M., Cerdan E., Gonzalez Siso M.I., Heterologous Kluyveromyces lactis β-galactosidase production and release by Saccharomyces cerevisiae osmotic-remedial thermosensitive autolytic mutants, Biochim. Biophys. Acta., 1335, pp. 235-241, (1997)

[8]

Becerra M., Cerdan E., Gonzalez Siso M.I., Micro-scale purification of β-galactosidase from Kluyveromyces lactis reveals that dimeric and tetrameric forms are active, Biotechnol.Techniques, 12, pp. 253-256, (1998)

[9]

De Nader Macias M.E., Perdigon G., Oliver G., De Ruiz Holgado A.A.P., Immunological relationships among β-galactosidases in members of the genus Lactobacillus, Int. J. Sys. Bacteriol., 35, pp. 103-110, (1985)

[10]

Guarante L., Yeast promoters and LacZ fusions designed to study expression of cloned genes in yeasts, Meth. Enzymol., 101, pp. 181-189, (1983)

← 1 2 →