UFL1 regulates milk protein and fat synthesis–related gene expression of bovine mammary epithelial cells probably via the mTOR signaling pathway

被引：0
作者：
Chengmin Li
Lian Li
Ilyas Ali
Meiqian Kuang
Xinling Wang
Genlin Wang
机构：
[1] Nanjing Agricultural University,College of Animal Science and Technology
[2] Jiangsu University of Science and Technology,Jiangsu Key Laboratory of Sericutural Biology and Biotechnology, School of Biotechnology
来源：
In Vitro Cellular & Developmental Biology - Animal | 2021年 / 57卷
关键词：
UFL1; Bovine mammary epithelial cells; Milk synthesis; Cell proliferation; mTOR;
D O I：
暂无
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学科分类号：
摘要：
UFL1 is an ufmylation (a novel post-translational modification) E3 ligase, mainly located in the endoplasmic reticulum (ER), that has emerged as a significant regulator of several physiological and pathological processes. Yet its physiological function in milk synthesis in bovine mammary epithelial cells (BMECs) remains unknown. In this study, we investigated the effects of UFL1 in milk protein and fat synthesis–related gene expression, with a particular emphasis on the role of UFL1 in LPS-treated BMECs. Results showed that UFL1 depletion significantly reduced the expression of milk protein and fat synthesis–related gene and mTOR phosphorylation in both normal and LPS-treated BMECs. Overexpression of UFL1 enhanced the activation of the mTOR and milk protein and fat synthesis–related gene expression. Collectively, these above results strongly demonstrate that UFL1 could regulate milk protein and fat synthesis–related gene expression of BMECs probably via the mTOR signaling pathway.
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页码：550 / 559
页数：9
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