Distinct hepatic lipid profile of hypertriglyceridemic mice determined by easy ambient sonic-spray ionization mass spectrometry

被引：0

作者：

Luciane C. Alberici

Helena C. F. Oliveira

Rodrigo R. Catharino

Anibal E. Vercesi

Marcos N. Eberlin

Rosana M. Alberici

机构：

[1] University of São Paulo,Department of Physics and Chemistry, Faculty of Pharmaceutical Sciences

[2] USP,Physiology and Biophysics Division, Biology Institute

[3] University of Campinas,Department of Clinical Pathology, Faculty of Medical Sciences

[4] UNICAMP,ThoMSon Mass Spectrometry Laboratory, Institute of Chemistry

[5] University of Campinas,undefined

[6] UNICAMP,undefined

[7] University of Campinas,undefined

[8] UNICAMP,undefined

来源：

Analytical and Bioanalytical Chemistry | 2011年 / 401卷

关键词：

Hypertriglyceridemia; Mice liver; Ambient ionization; Mass spectrometry; Lipids;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

Easy ambient sonic-spray ionization mass spectrometry (EASI-MS) was used to interrogate the hepatic lipid profiles of hypertriglyceridemic and control normotriglyceridemic mice. The analyses of ex vivo complex lipid mixtures were made directly with EASI-MS without accompanying separation steps. Intense ions for phosphatidylcholines and triacylglycerols were observed in the positive ion mode whereas the spectra in the negative ion mode provided profiles of phosphatidylethanolamines and phosphatidylinositol. EASI-MS was coupled to high-performance thin-layer chromatography for analysis of free fatty acids. Fourier transform–ion cyclotron resonance–mass spectrometry was also employed to confirm the identity of the detected lipids. We demonstrated higher incorporation of oleic acid in phosphatidylcholine and triacylglycerol composition, higher relative abundance of arachidonic acid containing phosphatidylinositol, and overall distinct free fatty acid profile in the livers of genetic hypertriglyceridemic mice. We propose that these alterations in liver lipid composition are related to the higher tissue and body metabolic rates described in these hypertriglyceridemic mice.

引用

页码：1651 / 1659

页数：8

共 216 条

[1]

Grundy SM(2004)Definition of metabolic syndrome: report of the National Heart, Lung, and Blood Institute/American Heart Association conference on scientific issues related to definition Circulation 109 433-438

[2]

Brewer HB(1999)Association of nonalcoholic fatty liver disease with insulin resistance Am J Med 107 450-455

[3]

Cleeman JI(2001)Nonalcoholic steatohepatitis Gastroenterology 121 710-723

[4]

Smith SC(1990)Hypertriglyceridemia as a result of human apolipoprotein C-III gene expression in transgenic mice Science 1249 790-793

[5]

Lenfant C(1992)Mechanism of hypertriglyceridemia in human apolipoprotein (apo) C-III transgenic mice: diminished very low density lipoprotein fractional catabolic rate associated with increased apoCIII and reduced apoE on the particles J Clin Invest 90 1889-1900

[6]

Marchesini G(1994)Overexpression of human apolipoprotein C-III in transgenic mice results in accumulation of apolipoprotein B48 remnant that is corrected by excess apolipoprotein E J Biol Chem 269 2324-2335

[7]

Brizi M(2009)Mitochondrial ATP-sensitive K(+) channels as redox signals to liver mitochondria in response to hypertriglyceridemia Free Radic Biol Med 47 1432-1439

[8]

Morselli-Labate AM(2003)Hypertriglyceridemia increases mitochondrial resting respiration and susceptibility to permeability transition J Bioenerg Biomembr 35 451-457

[9]

Bianchi G(2006)Hyperlipidemic mice present enhanced catabolism and higher mitochondrial ATP-sensitive K + channel activity Gastroenterology 131 1228-1234

[10]

Bugianesi E(2003)Global analyses of cellular lipidomes directly from crude extracts of biological samples by ESI mass spectrometry: a bridge to lipidomics J Lipid Res 44 1071-1079

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